Molecular recognition of flunarizine dihydrochloride and β-cyclodextrin inclusion complex by NMR and computational approaches

Flunarizine dihydrochloride (FLN) is used in the prophylactic treatment of migraine, vertigo, occlusive peripheral vascular disease and epilepsy. Cyclodextrins (CDs) are chiral, truncated cone shaped macrocycles known for their inner hydrophobic and outer hydrophilic site. They form complexes with hydrophobic drug molecules and enhance the solubility and bioavailability of such compounds by enhancing drug permeability through mucosal tissues. NMR spectroscopy and computational docking have been recognized as an important tool for the interaction study of CDs-drug inclusion complexes in solution state. The structural assignments of FLN and beta-CD protons were determined by H-1 NMR and 2D H-1-H-1 COSY NMR spectroscopy. H-1 NMR spectroscopic studies of FLN, beta-CD and their mixtures confirmed the formation of beta-CD-FLN inclusion complex in solution. H-1 NMR titration data for beta-CD-FLN inclusion complex showed 1:1 stoichiometry, an association constant of K (a) = 157 M-1 and change in Gibbs free energy of a dagger G = - 12.65 kJ mol(-1). The binding constant of the beta-CD inclusion complex with two nearly similar structures, FLN and cetirizine dihydrochloride, were compared. Two-dimensional H-1-H-1 ROESY spectral data and molecular docking studies showed the modes of penetration of the aromatic rings from the wider rim side into the beta-CD cavity. The possible geometrical structures of the beta-CD-FLN inclusion complex have been proposed in which aromatic rings protrude close to the narrower rim of the beta-CD truncated cone. NMR spectroscopic studies of FLN, beta-CD and FLN:beta-CD mixtures confirmed the formation of 1:1 inclusion complex in solution at room temperature. Two-dimensional H-1-H-1 ROESY together with molecular docking study confirmed that the F-substituted aromatic ring of FLN penetrates into beta-CD truncated cone and the tail of aromatic rings were proximal to narrower rim of beta-CD. The splitting of aromatic signals of FLN in the presence of beta-CD suggests chiral differentiation of the guest FLN by beta-CD.