Behavior of silk protein at the air-water interface

被引:36
作者
Yang, Yuhong [1 ]
Dicko, Cedric [2 ]
Bain, Colin D. [3 ]
Gong, Zuguang [4 ]
Jacobs, Robert M. J. [5 ]
Shao, Zhengzhong [4 ]
Terry, Ann E. [2 ]
Vollrath, Fritz [2 ]
机构
[1] Fudan Univ, Res Ctr Anal & Measurement, Shanghai 200433, Peoples R China
[2] Lund Univ, Ctr Chem, Pure & Appl Biochem Div, S-22100 Lund, Sweden
[3] Univ Durham, Dept Chem, Durham DH1 3LE, England
[4] Fudan Univ, Dept Macromol Sci, Adv Mat Lab, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[5] Univ Oxford, Chem Res Lab, Dept Chem, Oxford OX1 3TA, England
关键词
DYNAMIC SURFACE-TENSION; SOFT GLASSY MATERIALS; BOMBYX-MORI; RHEOLOGICAL PROPERTIES; ADSORPTION-KINETICS; AIR/WATER INTERFACE; GLOBULAR-PROTEINS; CRYSTAL-STRUCTURE; DROP VOLUME; FIBROIN;
D O I
10.1039/c2sm26054a
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The inability to link silk biomacromolecules' activity to their self-assembly and further fiber formation has limited a true implementation of a silk biotechnology. In this paper, we describe the application of video-enhanced drop shape analysis and interfacial shear rheological measurement to characterize the dynamic surface activity and interfacial interactions, as well as molecular structure within the interfacial layer of silk proteins. Quantitative analysis of the dynamic surface tension highlights two major mechanisms: (i) a mixed model at low concentration, and, (ii) a diffusion limited model at higher concentration. Once regenerated silk fibroin (RSF) is adsorbed at the air-water interface, interfacial gel-like structures are formed. The interfacial elastic modulus (G') of the adsorbed membranes exhibited a non-monotonic concentration dependence with a local maximum value at 1.0 x 10(-5) g mL(-1), indicating a different surface structure formed in RSF solution. The viscoelastic behavior varies with RSF concentration corresponding to three states of a soft glassy system, i. e. below, above and near the glass transition. A structural model for RSF adsorbed layers at the air-water interface at different bulk concentrations is suggested.
引用
收藏
页码:9705 / 9712
页数:8
相关论文
共 50 条
  • [21] Dynamics of amphiphilic diblock copolymers at the air-water interface
    Stocco, Antonio
    Tauer, Klaus
    Pispas, Stergios
    Sigel, Reinhard
    JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2011, 355 (01) : 172 - 178
  • [22] Mass spectrometry at the air-water interface
    Zhang, Xinxing
    INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2021, 462
  • [23] Crystallization of a polyphosphoester at the air-water interface
    Hasan, Nazmul
    Schwieger, Christian
    Tee, Hisaschi T.
    Wurm, Frederik R.
    Busse, Karsten
    Kressler, Joerg
    EUROPEAN POLYMER JOURNAL, 2018, 101 : 350 - 357
  • [24] Protein Adsorption at the Air-Water Interface by a Charge Sensing Interferometric Technique
    Brocca, Paola
    Saponaro, Andrea
    Introini, Bianca
    Rondelli, Valeria
    Pannuzzo, Martina
    Raciti, Domenica
    Corti, Mario
    Raudino, Antonio
    LANGMUIR, 2019, 35 (49) : 16087 - 16100
  • [25] Protein Adsorption at the Electrified Air-Water Interface: Implications on Foam Stability
    Engelhardt, Kathrin
    Rumpel, Armin
    Walter, Johannes
    Dombrowski, Jannika
    Kulozik, Ulrich
    Braunschweig, Bjoern
    Peukert, Wolfgang
    LANGMUIR, 2012, 28 (20) : 7780 - 7787
  • [26] Chiral recognition at the air-water interface
    Ariga, Katsuhiko
    Michinobu, Tsuyoshi
    Nakanishi, Takashi
    Hill, Jonathan P.
    CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2008, 13 (1-2) : 23 - 30
  • [27] Initial Conformation of Adsorbed Proteins at an Air-Water Interface
    Yano, Yohko F.
    Arakawa, Etsuo
    Voegeli, Wolfgang
    Kamezawa, Chika
    Matsushita, Tadashi
    JOURNAL OF PHYSICAL CHEMISTRY B, 2018, 122 (17) : 4662 - 4666
  • [28] Surface Properties and Conformation of Nephila clavipes Spider Recombinant Silk Proteins at the Air-Water Interface
    Renault, Anne
    Rioux-Dube, Jean-Francois
    Lefevre, Thierry
    Pezennec, Stephane
    Beaufils, Sylvie
    Vie, Veronique
    Tremblay, Melanie
    Pezolet, Michel
    LANGMUIR, 2009, 25 (14) : 8170 - 8180
  • [29] Atmospheric Intermediates at the Air-Water Interface
    Enami, Shinichi
    Numadate, Naoki
    Hama, Tetsuya
    JOURNAL OF PHYSICAL CHEMISTRY A, 2024, 128 (28) : 5419 - 5434
  • [30] Effect of enzymatic hydrolysis and polysaccharide addition on the β-lactoglobulin adsorption at the air-water interface
    Perez, Adrian A.
    Carrera Sanchez, Cecilio
    Rodriguez Patino, Juan M.
    Rubiolo, Amelia C.
    Santiago, Liliana G.
    JOURNAL OF FOOD ENGINEERING, 2012, 109 (04) : 712 - 720