Live-cell imaging of lipid droplets using solvatochromic coumarin derivatives

被引：17

作者：

Jana, Palash ^{[1
]}

Siva, Aravintha ^{[2
]}

Soppina, Virupakshi ^{[2
]}

Kanvah, Sriram ^{[1
]}

机构：

[1] Indian Inst Technol Gandhinagar, Dept Chem, Palaj 382355, Gandhinagar, India

[2] Indian Inst Technol Gandhinagar, Dept Biol Engn, Palaj 382355, Gandhinagar, India

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2020年 / 18卷 / 29期

关键词：

EMERGING ROLES; RED; BIOGENESIS; ORGANELLE; EMISSION; BIOLOGY; PROBES;

D O I：

10.1039/d0ob01277g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Lipid droplets (LDs), the lipid-rich intracellular organelles, serve to regulate many physiological processes and therefore attention has been attracted towards their selective detection. We report positively solvatochromic lipophilic dyes, based on the push-pull framework containing coumarin-pyridine heterocycles for selective live-cell imaging of lipid droplets (LDs) in Cos-7 and McA-RH7777 cells at ultralow concentrations (200 nM). The fluorescent probes show a remarkable increase in fluorescence intensity with time with the hydrophobic core of the lipid droplets contributing to the observed intensity enhancement. The simple structural framework, red emission, strong Stokes shift (>80 nm), and excellent biocompatibility highlight their significance as a versatile imaging tool for studying lipid droplets (LDs).

引用

页码：5608 / 5616

页数：9

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