Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins

被引:43
作者
Aremu, Adeyemi O. [1 ]
Gruz, Jiri [2 ,3 ,4 ]
Subrtova, Michaela [2 ,3 ,4 ]
Szuecova, Lucie [2 ,3 ,4 ]
Dolezal, Karel [2 ,3 ,4 ]
Bairu, Michael W. [1 ]
Finnie, Jeffrey F. [1 ]
Van Staden, Johannes [1 ]
机构
[1] Univ KwaZulu Natal, Sch Life Sci, Res Ctr Plant Growth & Dev, ZA-3209 Pietermaritzburg, Scottsville, South Africa
[2] Palacky Univ, Lab Growth Regulators, Olomouc 78371, Czech Republic
[3] Inst Expt Bot AS CR, Olomouc 78371, Czech Republic
[4] Palacky Univ, Fac Sci, Ctr Reg Hana Biotechnol & Agr Res, Olomouc 78371, Czech Republic
关键词
Conservation; Medicinal plants; Micropropagation; Phytochemicals; Topolins; BIOLOGICAL-ACTIVITY; VITRO; MICROPROPAGATION; FACTORIES; TUBERS; WILD;
D O I
10.1016/j.jplph.2013.04.008
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC-MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9 mu g g(-1)) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection. (C) 2013 Elsevier GmbH. All rights reserved.
引用
收藏
页码:1303 / 1308
页数:6
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