Determination of omeprazole in rat plasma by high-performance liquid chromatography without solvent extraction

被引：19

作者：

Jia, Huijuan ^{[1
]}

Li, Wei ^{[1
]}

Zhao, Kang ^{[1
]}

机构：

[1] Tianjin Univ, Coll Pharmaceut & Biotechnol, Tianjin 300072, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 837卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

omeprazole; rat plasma; high-performance liquid chromatography;

D O I：

10.1016/j.jchromb.2006.04.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC method without solvent extraction and using ultraviolet detection at 302 nm for the determination of omeprazole in rat plasma has been validated. Plasma samples after pretreatment with acetonitrile to effect deproteinization were dried under N-2 at 40 degrees C and reconstituted with mobile phase. The standard calibration curve for omeprazole was linear (r(2) = 0.9999) over the concentration range of 0.02-3 mu g ml(-1). The intra- and inter-day assay variability range was 4.8-9.2% and 5.2-10.3% individually. This method has been successfully applied to a pharmacokinetic study of omeprazole in rats. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：112 / 115

页数：4

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