Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes

被引：16

作者：

Malecka, Kamila ^{[1
]}

Grabowska, Iwona ^{[1
]}

Radecki, Jerzy ^{[1
]}

Stachyra, Anna ^{[2
]}

Gora-Sochacka, Anna ^{[2
]}

Sirko, Agnieszka ^{[2
]}

Radecka, Hanna ^{[1
]}

机构：

[1] Polish Acad Sci, Inst Anim Reprod & Food Res, PL-10748 Olsztyn, Poland

[2] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 08期

关键词：

Electrochemical genosensor; Amino-ssDNA probe; PCR products; Avian Influenza Virus; Gold electrode; LABEL-FREE DETECTION; DNA HYBRIDIZATION; VOLTAMMETRIC DETECTION; CARBON NANOTUBES; BIOSENSOR; SEQUENCE; IMMOBILIZATION; ACID;

D O I：

10.1002/elan.201300113

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A DNA biosensor for the detection of specific oligonucleotide sequences of Avian Influenza Virus type H5N1 has been proposed. The NH2-ssDNA probe was deposited onto a gold electrode surface to form an amide bond between the carboxyl group of thioacid and the amino group from ssDNA probe. The signals generated as a result of hybridization were registered in square wave voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)(6)](3-/4-) as a redox marker. The genosensor is capable to determine 20-mer and 180-bp (PCR products) oligonucleotides complementary sequences with detection limit in the fM range. The genosensor displays good selectivity and sensitivity. The 20-mer as well as 180-bp oligonucleotides without a complementary sequence generate very low signal.

引用

页码：1871 / 1878

页数：8

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