A set of polymorphic nuclear intron markers for conservation genetics and phylogeography of Euphorbia species (Pedilanthus clade)

被引：11

作者：

Garrick, R. C. ^{[1
]}

Meadows, C. A. ^{[1
]}

Nicolas, A. N. ^{[1
]}

Nason, J. D. ^{[2
]}

Dyer, R. J. ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA

[2] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA

来源：

CONSERVATION GENETICS | 2008年 / 9卷 / 06期

基金：

美国国家科学基金会;

关键词：

Co-dominant loci; Euphorbiaceae; Population structure; Phylogeny; Sonoran Desert;

D O I：

10.1007/s10592-008-9521-5

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We developed seven nuclear intron markers for Euphorbia lomelii. New exon-primed intron-crossing (EPIC) oligonucleotides were used for initial amplification and sequencing, then locus-specific primers and restriction-fragment-length polymorphism genotyping assays were designed. Loci showed no significant deviation from Hardy-Weinberg and linkage equilibrium, and they cross-amplify in at least three congeneric species.

引用

页码：1673 / 1676

页数：4

共 6 条

[1] Arlequin (version 3.0): An integrated software package for population genetics data analysis [J].

Excoffier, Laurent ;

Laval, Guillaume ;

Schneider, Stefan .

EVOLUTIONARY BIOINFORMATICS, 2005, 1 :47-50

[2] Phylogenetic utility of the nuclear gene malate synthase in the palm family (Arecaceae) [J].

Lewis, CE ;

Doyle, JJ .

MOLECULAR PHYLOGENETICS AND EVOLUTION, 2001, 19 (03) :409-420

[3] Extinction threat in the Pedilanthus clade (Euphorbia Euphorbiaceae) with special reference to the recently rediscovered E conzattii (P pulchellus) [J].

Olson, ME ;

Lomelí, SJA ;

Cacho, NI .

AMERICAN JOURNAL OF BOTANY, 2005, 92 (04) :634-641

[4] SINGLE-BASE SUBSTITUTIONS ARE DETECTED BY DOUBLE-STRAND CONFORMATION ANALYSIS [J].

SAAD, FA ;

HALLIGER, B ;

MULLER, CR ;

ROBERTS, RG ;

DANIELI, GA .

NUCLEIC ACIDS RESEARCH, 1994, 22 (20) :4352-4353

[5] HYDROCARBONS FROM PEDILANTHUS-MACROCARPUS (EUPHORBIACEAE) OF BAJA CALIFORNIA AND SONORA, MEXICO [J].

STERNBURG, C ;

RODRIGUEZ, E .

AMERICAN JOURNAL OF BOTANY, 1982, 69 (02) :214-218

[6] NEBcutter: a program to cleave DNA with restriction enzymes [J].

Vincze, T ;

Posfai, J ;

Roberts, RJ .

NUCLEIC ACIDS RESEARCH, 2003, 31 (13) :3688-3691

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