Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome

被引：274

作者：

Zhang, Yajie ^{[1
]}

Wang, Jianqi ^{[1
]}

Ding, Ming ^{[1
]}

Yu, Yonghao ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 10期

关键词：

TANDEM MASS-SPECTROMETRY; DNA-DAMAGE; PARP; CELLS; POLY(ADP-RIBOSYL)ATION; PHOSPHORYLATION; CHROMATOGRAPHY; INHIBITORS; INIPARIB; DOMAINS;

D O I：

10.1038/nmeth.2603

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.

引用

页码：981 / +

页数：6

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