Combined efficacy of clarithromycin plus cefazolin or vancomycin against Staphylococcus aureus biofilms formed on titanium medical devices

被引：33

作者：

Fujimura, Shigeru ^{[1
]}

Sato, Tetsuro ^{[2
]}

Mikami, Takeshi ^{[3
]}

Kikuchi, Toshiaki ^{[4
]}

Gomi, Kazunori ^{[4
]}

Watanabe, Akira ^{[1
]}

机构：

[1] Tohoku Univ, Inst Dev Aging & Canc, Res Div Dev Antiinfect Agents, Sendai, Miyagi 980, Japan

[2] Sendai Orthoped Hosp, Dept Orthoped, Sendai, Miyagi, Japan

[3] Tohoku Pharmaceut Univ, Dept Microbiol, Sendai, Miyagi, Japan

[4] Tohoku Univ, Dept Resp Med, Inst Dev Aging & Canc, Sendai, Miyagi 980, Japan

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2008年 / 32卷 / 06期

关键词：

Staphylococcus aureus; Biofilm; Medical device; Combined treatment;

D O I：

10.1016/j.ijantimicag.2008.06.030

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In this study, we investigated the in vitro efficacy of clarithromycin (CLA) combined with cefazolin (CFZ) or vancomycin ( VCM) against Staphylococcus aureus biofilms formed on titanium devices in order to confirm the efficacy of eradication therapies against device-related infection. The distribution of CLA in muscle tissue surrounding bone was also investigated by liquid chromatography/tandem mass spectrometry in 10 orthopaedic patients. Biofilm formation and eradication of S. aureus were monitored by scanning electron microscopy and using double-staining dyes, respectively. Although S. aureus biofilms were not eradicated by CLA, CFZ or VCM alone, CLA combined with CFZ or VCM destroyed biofilms, and S. aureus eradication was clearly observed 72 h later. This in vitro study showed that treatment with CLA plus CFZ or VCM destroyed staphylococcal biofilms formed on medical devices and eradicated S. aureus. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：481 / 484

页数：4

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