Biomarkers of liver fibrosis detecting with electrochemical immunosensor on clinical serum

被引：17

作者：

Yao, Yao ^{[1
]}

Bao, Jianfeng ^{[2
]}

Lu, Yanli ^{[1
]}

Zhang, Diming ^{[1
]}

Luo, Senbiao ^{[3
]}

Cheng, Xing ^{[1
]}

Zhang, Qian ^{[1
]}

Li, Shuang ^{[1
]}

Liu, Qingjun ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Biomed Engn, Educ Minist, Biosensor Natl Special Lab,Key Lab Biomed Engn, Hangzhou 310027, Zhejiang, Peoples R China

[2] Xixi Hosp Hangzhou, Hangzhou 310023, Zhejiang, Peoples R China

[3] Shangyu Peoples Hosp Zhejiang Prov, Shangyu 312000, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 222卷

基金：

中国国家自然科学基金;

关键词：

Liver fibrosis; Biosensor; Serum biomarker; Human transforming growth factor beta 1; Hyaluronate acid; Impedance; GROWTH-FACTOR-BETA; HEPATOCELLULAR-CARCINOMA; CARDIAC BIOMARKERS; HEPATITIS-C; MARKERS; PROGRESSION; ANALYTES; DISEASE; BINDING;

D O I：

10.1016/j.snb.2015.08.064

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Diagnosing hepatic fibrosis at an early stage with sensitive and specific monitoring approach is crucial for patient therapeutics and survival. In this study, an electrochemical immunosensor was established to detect representative biomarkers of liver fibrosis, such as hyaluronate acid (HA) and transforming growth factor beta 1 (TGF beta 1). Through a self-assembled monolayer of polyethylene glycol (PEG), antibodies against HA and TGF beta 1 were successfully immobilized on interdigitated electrodes. It produced a robust and sensitive membrane by improving the uniformity, density, and distribution of the antibodies for the biomarkers. Based on impedance sensing, HA and TGF beta 1 were sensitively detected in the ranges of 1-1000 ng/ml. The detection limits of HA and TGF beta 1 reached 0.586 ng/ml and 0.570 ng/ml, respectively. In addition, for the detection of clinical serum samples, the results were in excellent agreement with the tests of HA, type III pre-collagen (PCIII), IV collagen (IV-C), and laminin (LN) that conducted by radioimmunoassay for liver fibrosis. The research indicated that the approach provided a valuable, universal, and label-free strategy in evaluating liver fibrosis and other chronic diseases for point-of-care diagnostics. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：127 / 132

页数：6

共 32 条

[31] Ratiometric Fluorescent Biosensor for Hyaluronidase with Hyaluronan As Both Nanoparticle Scaffold and Substrate for Enzymatic Reaction
Xie, Huafei
Zeng, Fang
Wu, Shuizhu
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