Involvement of, CCL18 in. allergic asthma

被引:73
作者
De Nadai, Patricia
Charbonnier, Anne-Sophie
Chenivesse, Ccile
Senechal, Stephanie
Fournier, Clement
Gilet, Jules
Vorng, Han
Chang, Ying
Gosset, Philippe
Wallaert, Benoit
Tonnel, Andre-Bernard
Lassalle, Philippe
Tsicopoulos, Anne
机构
[1] Inst Natl Sante & Rech Med, U774, Inst Pasteur, F-59019 Lille, France
[2] Ctr Hosp Reg & Univ Lille, F-59037 Lille, France
[3] Clin Malad Resp, Lille, France
[4] Jilin Univ, Dept Cent Res, Clin Coll 3, Jilin, Peoples R China
关键词
D O I
10.4049/jimmunol.176.10.6286
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Allergic asthma is associated with a pulmonary recruitment of Th type 2 cells, basophils, and eosinophils, mainly linked to chemokine production. CCL18 is a chemokine preferentially expressed in the lung, secreted by APCs, induced by Th2-type cytokines, and only present in humans. Therefore, CCL18 may be involved in allergic asthma. PBMC from asthmatics allergic to house dust mite cultured in the presence of Dermatophagoide spteronyssinus 1 (Der p 1) allergen secreted CCL18, 48 and 72 h after stimulation, whereas those from healthy donors did not. Part of CCL18 was directly derived from Der p I-stimulated plasmacytoid dendritic cells, whereas the other part was linked to monocyte activation by IL-4 and IL-13 produced by Der p I-stimulated T cells. In bronchoalveolar lavages from untreated asthmatic allergic patients, CCL18 was highly increased compared with controls. Functionally, CCL18 preferentially attracted in vitro-polarized Th2 cells and basophils, but not eosinophils and Th1 cells, and induced basophil histamine and intracellular calcium release. These data show a new function for CCL18, i.e., the recruitment of Th2 cells and basophils, and suggest that CCL18 may play a predominant role in allergic asthma.
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页码:6286 / 6293
页数:8
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