Establishment of a humanized model of liver using NOD/Shi-scid IL2Rgnull mice

被引:89
作者
Suemizu, Hiroshi [1 ]
Hasegawa, Masami [1 ,4 ]
Kawai, Kenji [2 ]
Taniguchi, Kenji [1 ,4 ]
Monnai, Makoto [1 ,5 ]
Wakui, Masatoshi [2 ,6 ]
Suematsu, Makoto [6 ]
Ito, Mamoru [3 ]
Peltz, Gary [7 ]
Nakamura, Masato [2 ,8 ]
机构
[1] Cent Inst Expt Anim, Biomed Res Dept, Kanagawa 2160001, Japan
[2] Cent Inst Expt Anim, Dept Pathol Res, Kanagawa 2160001, Japan
[3] Cent Inst Expt Anim, Lab Anim Res Dept, Kanagawa 2160001, Japan
[4] Chugai Pharmaceut Co Ltd, Pharmaceut Res Dept 2, Kanagawa 2478530, Japan
[5] Chugai Res Inst Med Sci Inc, Kanagawa 2478530, Japan
[6] Keio Univ, Sch Med, Dept Biochem & Integrat Med Biol, Shinjuku Ku, Tokyo 1608582, Japan
[7] Roche Palo Alto, Dept Genet & Genom, Palo Alto, CA 94304 USA
[8] Tokai Univ, Sch Med, Dept Pathol, Kanagawa 2591193, Japan
关键词
Humanized mouse; Liver repopulation; NOD/Shi-scid IL2Rg(null) (NOG) mouse; Urokinase-type plasminogen activator;
D O I
10.1016/j.bbrc.2008.09.124
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Severely immunodeficient NOD/Shi-scid IL2Rg(null) (NOG) mice are used as recipients for human tissue transplantation, which produces chimeric mice with various types Of human tissue. NOG mice expressing transgenic Urokinase-type plasminogen activator in the liver (uPA-NOG) were produced. Human hepatocytes injected into uPA-NOG mice repopulated the recipient livers with human cells. The uPA-NOG model has several advantages over previously produced chimeric mouse models of human liver: (1) the severely immunodeficient NOG background enables higher xenogeneic cell engraftment; (2) the absence of neonatal lethality enables mating of homozygotes, which increased the efficacy of homozygote production: and (3) donor xenogeneic human hepatocytes Could be readily transplanted into Young uPA-NOG mice, which provide easier surgical manipulation and improved recipient Survival. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:248 / 252
页数:5
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