HY5 regulates anthocyanin biosynthesis by inducing the transcriptional activation of the MYB75/PAP1 transcription factor in Arabidopsis

被引：346
作者：
Shin, Dong Ho [1 ]
Choi, MyungGoo [1 ]
Kim, Keunhwa [2 ]
Bang, Geul [1 ]
Cho, Misuk [1 ]
Choi, Sang-Bong [3 ]
Choi, Giltsu [2 ]
Park, Youn-Il [1 ]
机构：
[1] Chungnam Natl Univ, Dept Biol Sci, Taejon 305764, South Korea
[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea
[3] Myongji Univ, Div Biosci & Bioinformat, Yongin 449728, South Korea
来源：
FEBS LETTERS | 2013年 / 587卷 / 10期
关键词：
Anthocyanin biosynthesis; Arabidopsis; HY5; PAP1; BZIP PROTEIN; GENE; PROMOTERS; ENCODES;
D O I：
10.1016/j.febslet.2013.03.037
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are currently unknown. To address this problem, we analyzed crosses between hy5 and pap1 mutants (hy5pap1) or pap1D overexpressors (hy5pap1D), performed chromatin immunoprecipitation-qPCR, and determined the PAP1 promoter region through deletion analysis. The results show that HY5 regulates PAP1 expression via direct binding to G- and ACE-boxes in the promoter region, which suggests bifurcate regulation of anthocyanin biosynthesis by HY5 via transcriptional activation of PAP1. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
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页码：1543 / 1547
页数：5
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