HY5 regulates anthocyanin biosynthesis by inducing the transcriptional activation of the MYB75/PAP1 transcription factor in Arabidopsis

被引：322

作者：

Shin, Dong Ho ^{[1
]}

Choi, MyungGoo ^{[1
]}

Kim, Keunhwa ^{[2
]}

Bang, Geul ^{[1
]}

Cho, Misuk ^{[1
]}

Choi, Sang-Bong ^{[3
]}

Choi, Giltsu ^{[2
]}

Park, Youn-Il ^{[1
]}

机构：

[1] Chungnam Natl Univ, Dept Biol Sci, Taejon 305764, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[3] Myongji Univ, Div Biosci & Bioinformat, Yongin 449728, South Korea

来源：

FEBS LETTERS | 2013年 / 587卷 / 10期

关键词：

Anthocyanin biosynthesis; Arabidopsis; HY5; PAP1; BZIP PROTEIN; GENE; PROMOTERS; ENCODES;

D O I：

10.1016/j.febslet.2013.03.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are currently unknown. To address this problem, we analyzed crosses between hy5 and pap1 mutants (hy5pap1) or pap1D overexpressors (hy5pap1D), performed chromatin immunoprecipitation-qPCR, and determined the PAP1 promoter region through deletion analysis. The results show that HY5 regulates PAP1 expression via direct binding to G- and ACE-boxes in the promoter region, which suggests bifurcate regulation of anthocyanin biosynthesis by HY5 via transcriptional activation of PAP1. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1543 / 1547

页数：5

共 22 条

[1] HY4 GENE OF A-THALIANA ENCODES A PROTEIN WITH CHARACTERISTICS OF A BLUE-LIGHT PHOTORECEPTOR
AHMAD, M
CASHMORE, AR
[J]. NATURE, 1993, 366 (6451) : 162 - 166
[2] Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis
Borevitz, JO
Xia, YJ
Blount, J
Dixon, RA
Lamb, C
[J]. PLANT CELL, 2000, 12 (12) : 2383 - 2393
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression
Chattopadhyay, S
Ang, LH
Puente, P
Deng, XW
Wei, N
[J]. PLANT CELL, 1998, 10 (05) : 673 - 683
[5] Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana
Cominelli, Eleonora
Gusmaroli, Giuliana
Allegra, Domenico
Galbiati, Massimo
Wade, Helena K.
Jenkins, Gareth I.
Tonelli, Chiara
[J]. JOURNAL OF PLANT PHYSIOLOGY, 2008, 165 (08) : 886 - 894
[6] Identification of a cis-regulatory element by transient analysis of co-ordinately regulated genes
Dare, Andrew P.
Schaffer, Robert J.
Lin-Wang, Kui
Allan, Andrew C.
Hellens, Roger P.
[J]. PLANT METHODS, 2008, 4 (1)
[7] Sugar-hormone cross-talk in anthocyanin biosynthesis
Das, Prasanta Kumar
Shin, Dong Ho
Choi, Sang-Bong
Park, Youn-Il
[J]. MOLECULES AND CELLS, 2012, 34 (06) : 501 - 507
[8] Phenotypic characterization of a photomorphogenic mutant
Fankhauser, C
Casal, JJ
[J]. PLANT JOURNAL, 2004, 39 (05) : 747 - 760
[9] Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings
Gonzalez, Antonio
Zhao, Mingzhe
Leavitt, John M.
Lloyd, Alan M.
[J]. PLANT JOURNAL, 2008, 53 (05) : 814 - 827
[10] UV-B photoreceptor-mediated signalling in plants
Heijde, Marc
Ulm, Roman
[J]. TRENDS IN PLANT SCIENCE, 2012, 17 (04) : 230 - 237

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