Human Umbilical Cord Mesenchymal Stem Cells Support Nontumorigenic Expansion of Human Embryonic Stem Cells

被引：25

作者：

Ding, Dah-Ching ^{[1
,2
]}

Shyu, Woei-Cherng ^{[3
,4
]}

Lin, Shinn-Zong ^{[3
,4
,5
]}

Liu, Hwan-Wun ^{[2
,6
]}

Chiou, Shih-Hwa ^{[7
,8
]}

Chu, Tang-Yuan ^{[1
,2
]}

机构：

[1] Tzu Chi Univ, Buddhist Tzu Chi Gen Hosp, Dept Obstet & Gynecol, Hualien, Taiwan

[2] Tzu Chi Univ, Grad Inst Med Sci, Hualien, Taiwan

[3] China Med Univ & Hosp, Ctr Neuropsychiat, Taichung, Taiwan

[4] China Med Univ, Grad Inst Immunol, Taichung, Taiwan

[5] China Med Univ, Beigang Hosp, Yunlin, Taiwan

[6] Buddhist Tzu Chi Buddhist Gen Hosp, Dept Occupat Med, Hualien, Taiwan

[7] Natl Yang Ming Univ, Inst Clin Med, Inst Pharmacol, Taipei 112, Taiwan

[8] Taipei Vet Gen Hosp, Taipei, Taiwan

来源：

CELL TRANSPLANTATION | 2012年 / 21卷 / 07期

关键词：

Embryonic stem cells; Umbilical cord; Mesenchymal stem cells; Tumorigenic; TERATOMA FORMATION; HUMAN BLASTOCYSTS; UNDIFFERENTIATED GROWTH; MOUSE EMBRYOS; MATRIX CELLS; IN-VITRO; CULTURE; LINES; DIFFERENTIATION; TRANSPLANTATION;

D O I：

10.3727/096368912X647199

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The expansion of pluripotent human embryonic stem cells (hESCs) requires a culture on feeder layers of mouse embryonic fibroblasts (MEFs). The culture model often causes immunogenic contaminations such as xenocarbohydrate, and inevitably forms teratoma in vivo. This study tested human umbilical cord-derived mesenchymal stem cells (HUCMSCs) as the feeder for hESCs. Wharton's jelly-derived HUCMSCs showed characteristics of MSCs and were easily maintained in a culture for over 20 passages. Under the mitomycininhibited HUCMSC feeder, hESCs maintained the features of embryonic stem cells (pluripotency and maintenance of normal karyotypes) after a prolonged culture of more than 20 passages. Notably, in extensive trials, no teratoma was formed in xenograft in NOD/SCID mice, but subsequent resumption of teratoma formation was noted upon transient coculturing with MEFs. Interestingly, among the four pluripotencyconferring genes, MYC and OCT4 were found to be downregulated in hESCs cocultured with HUCMSCs. Results of this study supported a nontumorigenic sustained culture of hESCs and did not form teratoma in vivo.

引用

页码：1515 / 1527

页数：13

共 39 条

[21] Optimization of Culture Conditions to Support Undifferentiated Growth of Human Embryonic Stem Cells [J].