Apoptosis signal-regulating kinase (ASK1) and transcription factor tumor suppressor protein TP53 suppress rabbit ovarian granulosa cell functions

被引:10
作者
Sirotkin, Alexander, V [1 ,2 ]
Petrak, Juraj [3 ]
Alwasel, Saleh [4 ]
Harrath, Abdel Halim [4 ]
机构
[1] Constantine Philosopher Univ, Nitra 94974, Slovakia
[2] Res Inst Anim Prod Nitre, Luzianky 95141, Slovakia
[3] Slovak Univ Agr, Nara 94976, Japan
[4] King Saud Univ, Coll Sci, Dept Zool, Riyadh 11451, Saudi Arabia
关键词
TP53; ASK-1; Ovarian granulosa cells; Proliferation; Apoptosis; Rabbit; SECRETORY ACTIVITY; FACTOR P53; IGF-I; PROLIFERATION; LEPTIN;
D O I
10.1016/j.anireprosci.2019.03.018
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
This study was conducted with the aim to understand the roles of apoptosis signal-regulating kinase (ASK1) and transcription factor tumor suppressor protein TP53, as well as the possible interrelationships, in the control of healthy ovarian cell functions. Rabbit ovarian granulosa cells were transfected with constructs encoding ASK1, TP53, or TP53 + ASK1 and cultured with or without insulin-like growth factor 1 (IGF1). The accumulation of ASK1, the cytoplasmic apoptosis regulators SAX and BCL2, and proliferating cell nuclear antigen (PCNA, a cell proliferation marker), as well as progesterone release, were evaluated by quantitative immunocytochemistry and radioimmunoassay. Results indicate both ASK1 and TP53 promoted the accumulation of BAX, but suppressed that of BCL2 and PCNA. Progesterone release was inhibited by ASK1 and promoted by TP53, while TP53 also stimulated ASK1 accumulation. Additionally, IGF1 stimulated PCNA and reduced progesterone release, but did not affect ASK1. Transfection with ASK1, TP53, or TP53 + ASK1 could modify IGF1 activity, however, there was no cumulative effect with co-transfection of TP53 and ASK1. This is the first results that indicate there is ASK1 suppression of healthy ovarian granulosa cell functions, including promoting apoptosis, inhibiting proliferation, and alter progesterone release. There was also TP53 actions in rabbit ovarian granulosa cells, where it stimulated ASK1, apoptosis, and progesterone release, thus suppressing proliferation and responses to IGF1. The similarity of ASK1 and TP53 effects on apoptosis and proliferation, lack of cumulative action of these molecules, and capacity of TP53 to promote ASK1 accumulation suggest that TP53 can suppress some ovarian granulosa cell functions through ASK1 stimulation.
引用
收藏
页码:140 / 151
页数:12
相关论文
共 21 条
[1]   Insulin-Like Growth Factor (IGF) System in Liver Diseases [J].
Adamek, Agnieszka ;
Kasprzak, Aldona .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (05)
[2]   A systematic review of p53 regulation of oxidative stress in skeletal muscle [J].
Beyfuss, Kaitlyn ;
Hood, David A. .
REDOX REPORT, 2018, 23 (01) :100-117
[3]   The BCL-2 family of proteins and mitochondrial outer membrane permeabilisation [J].
Birkinshaw, Richard W. ;
Czabotar, Peter E. .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2017, 72 :152-162
[4]   The IGF-p53 connection in cancer [J].
Conover, Cheryl A. .
GROWTH HORMONE & IGF RESEARCH, 2018, 39 :25-28
[5]   How to unleash mitochondrial apoptotic blockades to kill cancers? [J].
Deng, Jing .
ACTA PHARMACEUTICA SINICA B, 2017, 7 (01) :18-26
[6]   Mutant p53 as a target for cancer treatment [J].
Duffy, Michael J. ;
Synnott, Naoise C. ;
Crown, John .
EUROPEAN JOURNAL OF CANCER, 2017, 83 :258-265
[7]   miR-106a Increases Granulosa Cell Viability and Is Downregulated in Women With Diminished Ovarian Reserve [J].
Hong, Liming ;
Peng, Sha ;
Li, Ying ;
Fang, Ying ;
Wang, Qin ;
Klausen, Christian ;
Yin, Chenghong ;
Wang, Shuyu ;
Leung, Peter C. K. ;
Yang, Xiaokui .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2018, 103 (06) :2157-2166
[8]   Insulin-like growth factor (IGF) axis in cancerogenesis [J].
Kasprzak, Aldona ;
Kwasniewski, Wojciech ;
Adamek, Agnieszka ;
Gozdzicka-Jozefiak, Anna .
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2017, 772 :78-104
[9]  
Liu Tingting, 2017, Advances in Biological Regulation, V66, P54, DOI 10.1016/j.jbior.2017.10.011
[10]   The role of IGF-I, cAMP/protein kinase A and MAP-kinase in the control of steroid secretion, cyclic nucleotide production, granulosa cell proliferation and preimplantation embryo development in rabbits [J].
Makarevich, A ;
Sirotkin, A ;
Chrenek, P ;
Bulla, J ;
Hetenyi, L .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2000, 73 (3-4) :123-133