Differential expression of progesterone receptor isoforms related to PGR+331g/a polymorphism in endometriosis: A case-control study

被引:0
作者
Mousazadeh, Sepideh [1 ,2 ]
Ghaheri, Azadeh [3 ]
Shahhoseini, Maryam [2 ]
Aflatoonian, Reza [4 ]
Afsharian, Parvaneh [2 ]
机构
[1] Iran Univ Med Sci, Fac Adv Technol Med, Dept Tissue Engn & Regenerat Med, Tehran, Iran
[2] ACECR, Royan Inst Reprod Biomed, Dept Genet, Reprod Biomed Res Ctr, Tehran, Iran
[3] ACECR, Royan Inst Reprod Biomed, Dept Epidemiol & Reprod Hlth, Reprod Epidemiol Res Ctr, Tehran, Iran
[4] ACECR, Royan Inst Reprod Biomed, Reprod Biomed Res Ctr, Dept Endocrinol & Female Infertil, Tehran, Iran
关键词
Endometriosis; Progesterone receptor A; Progesterone receptor B; rs10895068; RISK; GENE; INFERTILITY; ASSOCIATION; RESISTANCE; PROMOTER; ALPHA;
D O I
10.18502/4)97-28-3
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Background: Endometriosis are defined as a progesterone-resistance disease. Two progesterone receptor (PR) isoforms, namely PR-A and PR-B, mediate the special effects of progesterone. One of the most effective polymorphism in the promoter region of PGR is the +331G/A. Objective: The differential expression level of PR isoforms due to +331G/A polymorphism may be able to influence the function of progesterone and reduce the susceptibility of endometriosis. Materials and Methods: This analytic, case-control study was carried out at Royan Institute, Tehran, Iran. Whole-blood samples were collected from 98 infertile women undergoing laparoscopy for endometriosis and 102 healthy fertile women. After DNA extraction, genotype frequencies were determined by polymerase chain reactionrestriction fragment length polymorphism. Then, RNA was extracted from the selected eutopic tissue samples of endometriosis patients. Analysis of PR-A and PR-B mRNA expressions were performed using Real-time polymerase chain reaction. Results: The frequency distribution of GG, GA genotypes in +331G/A polymorphism was 98.04%, 1.96% in the patients and 97.96%, 2.04% in the control groups, respectively (p= 0.968). Although our data did not show any significant association with +331G/A in the patient and control groups, we were able to demonstrate significantly higher expression level of PR-B and no significant lower expression level of PR-A isoforms in patients by favoring GA to GG genotypes (p= 0.017, p= 0.731, respectively). Conclusion: Our findings show that patients with GA genotypes had a higher expression level of PR-B compared to patients with GG genotypes.
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页数:10
相关论文
共 30 条
[1]   The use of biochemical markers in the diagnosis of pelvic endometriosis [J].
Abrao, MS ;
Podgaec, S ;
Martorelli, B ;
Ramos, LO ;
Pinotti, JA ;
de Oliveira, RM .
HUMAN REPRODUCTION, 1997, 12 (11) :2523-2527
[2]   Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques [J].
Aljanabi, SM ;
Martinez, I .
NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4692-4693
[3]   Definitions of infertility and recurrent pregnancy loss: a committee opinion [J].
不详 .
FERTILITY AND STERILITY, 2013, 99 (01) :63-63
[4]   Progesterone receptor isoform A but not B is expressed in endometriosis [J].
Attia, GR ;
Zeitoun, K ;
Edwards, D ;
Johns, A ;
Carr, BR ;
Bulun, SE .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2000, 85 (08) :2897-2902
[5]  
Berchuck A, 2004, CANCER EPIDEM BIOMAR, V13, P2141
[6]   Endometriosis and infertility [J].
Bulletti, Carlo ;
Coccia, Maria Elisabetta ;
Battistoni, Silvia ;
Borini, Andrea .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2010, 27 (08) :441-447
[7]   Progesterone resistance in endometriosis: Link to failure to metabolize estradiol [J].
Bulun, SE ;
Cheng, YH ;
Yin, P ;
Imir, G ;
Utsunomiya, H ;
Attar, E ;
Innes, J ;
Kim, JJ .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 248 (1-2) :94-103
[8]  
Bulun Serdar E, 2010, Semin Reprod Med, V28, P44, DOI 10.1055/s-0029-1242992
[9]   Estrogen Receptor-β, Estrogen Receptor-α, and Progesterone Resistance in Endometriosis [J].
Bulun, Serdar E. ;
Cheng, You-Hong ;
Pavone, Mary Ellen ;
Xue, Qing ;
Attar, Erkut ;
Trukhacheva, Elena ;
Tokunaga, Hideki ;
Utsunomiya, Hiroki ;
Yin, Ping ;
Luo, Xia ;
Lin, Zhihong ;
Imir, Gonca ;
Thung, Stephen ;
Su, Emily J. ;
Kim, J. Julie .
SEMINARS IN REPRODUCTIVE MEDICINE, 2010, 28 (01) :36-43
[10]  
Chaudhary S, 2013, PLOS ONE, V8