Nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/chitosan scaffolds for skin regeneration

被引：76
作者：
Veleirinho, Beatriz [1 ]
Coelho, Daniela S. [2 ]
Dias, Paulo F. [2 ]
Maraschin, Marcelo [3 ]
Ribeiro-do-Valle, Rosa M. [4 ]
Lopes-da-Silva, Jose A. [1 ]
机构：
[1] Univ Aveiro, Dept Chem, QOPNA Res Unit, P-3810193 Aveiro, Portugal
[2] Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, BR-88040900 Florianopolis, SC, Brazil
[3] Univ Fed Santa Catarina, Dept Plant Sci, BR-88040900 Florianopolis, SC, Brazil
[4] Univ Fed Santa Catarina, Dept Pharmacol, BR-88040900 Florianopolis, SC, Brazil
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 51卷 / 04期
关键词：
PHBV; Chitosan; Electrospinning; Scaffold; Skin healing; IN-VITRO; CHITOSAN NANOPARTICLES; POLY; 3-HYDROXYBUTYRATE; FIBER MATS; ANTIBACTERIAL; POLYHYDROXYALKANOATES; BIOCOMPATIBILITY; INFLAMMATION; REPAIR; BLEND;
D O I：
10.1016/j.ijbiomac.2012.05.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this study was to evaluate hybrid poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitosan nanofibrous mats as scaffolds for skin engineering. In vitro studies were carried out to test the potential of the scaffolds for fibroblasts adhesion, viability, and proliferation (L929 cell line). The in vivo performance was also studied in a full-thickness wound healing model. PHBV/chitosan 4:1 (w/w) exhibited a higher in vitro biocompatibility and a better ability for cell adhesion and growth, compared to PHBV/chitosan 2:3 (w/w). The in vivo assay also revealed the better performance of this scaffold, improving the wound healing process in rats. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：343 / 350
页数：8
相关论文
共 31 条
[1]   Review - Properties of blends and composites based on poly(3-hydroxy)butyrate (PHB) and poly(3-hydroxybutyrate-hydroxyvalerate) (PHBV) copolymers [J].
Avella, M ;
Martuscelli, E ;
Raimo, M .
JOURNAL OF MATERIALS SCIENCE, 2000, 35 (03) :523-545
[2]   Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications [J].
Cai, Zeng-xiao ;
Mo, Xiu-mei ;
Zhang, Kui-hua ;
Fan, Lin-peng ;
Yin, An-lin ;
He, Chuang-long ;
Wang, Hong-sheng .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2010, 11 (09) :3529-3539
[3]   Geometric control of cell life and death [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
SCIENCE, 1997, 276 (5317) :1425-1428
[4]   The application of polyhydroxyalkanoates as tissue engineering materials [J].
Chen, GQ ;
Wu, Q .
BIOMATERIALS, 2005, 26 (33) :6565-6578
[5]   Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution [J].
Chong, E. J. ;
Phan, T. T. ;
Lim, I. J. ;
Zhang, Y. Z. ;
Bay, B. H. ;
Ramakrishna, S. ;
Lim, C. T. .
ACTA BIOMATERIALIA, 2007, 3 (03) :321-330
[6]   Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid [J].
Hu, SG ;
Jou, CH ;
Yang, MC .
BIOMATERIALS, 2003, 24 (16) :2685-2693
[7]   Antibacterial and biodegradable properties of polyhydroxyalkanoates grafted with chitosan and chitooligosaccharides via ozone treatment [J].
Hu, SG ;
Jou, CH ;
Yang, MC .
JOURNAL OF APPLIED POLYMER SCIENCE, 2003, 88 (12) :2797-2803
[8]   Toxicity evaluation of biodegradable chitosan nanoparticles using a zebrafish embryo model [J].
Hu, Yu-Lan ;
Qi, Wang ;
Han, Feng ;
Shao, Jian-Zhong ;
Gao, Jian-Qing .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2011, 6 :3351-3359
[9]   Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice [J].
Keylock, K. Todd ;
Vieira, Victoria J. ;
Wallig, Matthew A. ;
DiPietro, Luisa A. ;
Schrementi, Megan ;
Woods, Jeffrey A. .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2008, 294 (01) :R179-R184
[10]   Electrospun nanofiber scaffolds: engineering soft tissues [J].
Kumbar, S. G. ;
James, R. ;
Nukavarapu, S. P. ;
Laurencin, C. T. .
BIOMEDICAL MATERIALS, 2008, 3 (03)
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