Protein, carbohydrate, lipid and chlorophyll α content in Isochrysis aff. galbana (clone T-Iso) cultured with a low cost alternative to the f/2 medium

Growth, proximate composition and chlorophyll a content was estimated in static cultures of Isochrysis aff. galbana (clone T-Iso) to compare the quality and quantity of the biomass produced with an agricultural fertilizer medium versus f/2 medium. Culture of microalgae was done with controlled temperature, air flux of 4.5 1 min(-1), salinity of 33parts per thousand and an irradiance of 110 +/- 3 mumol m(-2) s(-1). Daily samples from the cultures were taken to estimate cellular density, proximate composition and chlorophyll a content during 7 days. Mean cellular density from samples with f/2 medium and with agricultural fertilizer were similar (no significance at P less than or equal to 0.05), and mean growth rates of 0.62 and 0.61 doubling per day, respectively. Maximum similar values of protein content of 7.3 and 7.6 pg per cell were estimated on the 5th day in fit and agricultural fertilizer medium, respectively. Carbohydrate and lipid content decreased during the first 5 days corresponding to the exponential growth in both experiments. Higher content of carbohydrate (no significance at P less than or equal to 0.05) was found in both media during the first 2 days, after 4 days the carbohydrates reduced three times theirs concentrations. Generally lipid contents in the cultures with agricultural fertilizer were significantly higher (P less than or equal to 0.05) than f/2 medium. The chlorophyll a content increased exponentially during the culture, and its concentration was similar in both experiments. In conclusion, the cellular density and the biochemical constituents were similar when using either media. This fertilizer can be used in the production of I. galbana (T-Iso) employed in aquaculture. (C) 2002 Elsevier Science B.V. All rights reserved.