In Situ Determination of Clostridium Endospore Membrane Fluidity during Pressure-Assisted Thermal Processing in Combination with Nisin or Reutericyclin

被引:17
作者
Hofstetter, S. [1 ]
Winter, R. [2 ]
McMullen, L. M. [1 ]
Gaenzle, M. G. [1 ]
机构
[1] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada
[2] Tech Univ Dortmund, Fak Chem, Dortmund, Germany
基金
加拿大自然科学与工程研究理事会;
关键词
BACILLUS-SUBTILIS; LACTOBACILLUS-REUTERI; INNER MEMBRANE; SPORES; INACTIVATION; RESISTANCE; TEMPERATURE; GERMINATION; MEGATERIUM; MIXTURES;
D O I
10.1128/AEM.03755-12
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This study determined the membrane fluidity of clostridial endospores during treatment with heat and pressure with nisin or reutericyclin. Heating (90 degrees C) reduced laurdan (6-dodecanoyl-2-dimethylaminonaphthalene) general polarization, corresponding to membrane fluidization. Pressure (200 MPa) stabilized membrane order. Reutericyclin and nisin exhibit divergent effects on heat- and pressure-induced spore inactivation and membrane fluidity.
引用
收藏
页码:2103 / 2106
页数:4
相关论文
共 28 条
[1]   BIOCHEMICAL STUDIES OF BACTERIAL SPORULATION AND GERMINATION .14. PHOSPHOLIPIDS IN BACILLUS MEGATERIUM [J].
BERTSCH, LL ;
BONSEN, PPM ;
KORNBERG, A .
JOURNAL OF BACTERIOLOGY, 1969, 98 (01) :75-&
[2]   Response of spores to high-pressure processing [J].
Black, Elaine P. ;
Setlow, Peter ;
Hocking, Ailsa D. ;
Stewart, Cynthia M. ;
Kelly, Alan L. ;
Hoover, Dallas G. .
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2007, 6 (04) :103-119
[3]   Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile [J].
Cowan, AE ;
Olivastro, EM ;
Koppel, DE ;
Loshon, CA ;
Setlow, B ;
Setlow, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (20) :7733-7738
[4]   Reutericyclin:: biological activity, mode of action, and potential applications [J].
Gänzle, MG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (03) :326-332
[5]   Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584 [J].
Gänzle, MG ;
Höltzel, A ;
Walter, J ;
Jung, G ;
Hammes, WP .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4325-4333
[6]   Killing of spores of Bacillus subtilis by peroxynitrite appears to be caused by membrane damage [J].
Genest, PC ;
Setlow, B ;
Melly, E ;
Setlow, P .
MICROBIOLOGY-SGM, 2002, 148 :307-314
[7]   Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order [J].
Harris, FM ;
Best, KB ;
Bell, JD .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2002, 1565 (01) :123-128
[8]  
Herberhold H, 2007, J MOL BIOL, V330, P1154
[9]   Effects of nisin and reutericyclin on resistance of endospores of Clostridium spp. to heat and high pressure [J].
Hofstetter, Simmon ;
Gebhardt, David ;
Ho, Linda ;
Gaenzle, Michael ;
McMullen, Lynn M. .
FOOD MICROBIOLOGY, 2013, 34 (01) :46-51
[10]   Use of the fluorescent probe LAURDAN to label and measure inner membrane fluidity of endospores of Clostridium spp. [J].
Hofstetter, Simmon ;
Denter, Christian ;
Winter, Roland ;
McMullen, Lynn M. ;
Gaenzle, Michael G. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 91 (01) :93-100