Target-Responsive "Sweet" Hydrogel with Glucometer Readout for Portable and Quantitative Detection of Non-Glucose Targets

被引：299

作者：

Yan, Ling ^{[1
,2
]}

Zhu, Zhi ^{[1
,2
]}

Zou, Yuan ^{[1
,2
]}

Huang, Yishun ^{[1
,2
]}

Liu, Dewen ^{[1
,2
]}

Jia, Shasha ^{[1
,2
]}

Xu, Dunming ^{[3
]}

Wu, Min ^{[3
]}

Zhou, Yu ^{[3
]}

Zhou, Shuang ^{[3
]}

Yang, Chaoyong James ^{[1
,2
]}

机构：

[1] Xiamen Univ, Key Lab Chem Biol Fujian Prov, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn,Key Lab Analyt, Xiamen 361005, Peoples R China

[2] Xiamen Univ, Coll Chem & Chem Engn, Dept Biol Chem, Xiamen 361005, Peoples R China

[3] Xiamen Entry Exit Inspect & Quarantine Bur, Xiamen 361026, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 10期

基金：

中国国家自然科学基金;

关键词：

ECGONINE METHYL-ESTER; MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; ASPERGILLUS-NIGER; BETA-AMYLASE; HUMAN HAIR; COCAINE; METABOLITES; POINT; CARE;

D O I：

10.1021/ja3114714

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number of quantitative portable devices are commercially available, among which the personal glucose meter (PGM) is the most successful example and has been the most widely used. However, PGMs can detect only blood glucose as the unique target. Here we describe a novel design that combines a glucoamylase-trapped aptamer-cross-linked hydrogel with a PGM for portable and quantitative detection of non-glucose targets. Upon target introduction, the hydrogel collapses to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose for quantitative readout by the PGM. With the advantages of low cost, rapidity, portability, and ease of use, the method reported here has the potential to be used by the public for portable and quantitative detection of a wide range of non-glucose targets.

引用

页码：3748 / 3751

页数：4

共 45 条

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