Insertionally inactivated and inducible recA alleles for use in Neisseria

被引:86
作者
Seifert, HS
机构
[1] NW University Medical School, Dept. of Microbiology-Immunology, Mailcode W213, Searle 6-467, Chicago, IL 60611
关键词
DNA recombination; lac operon; DNA transformation; Pilin variation; DNA repair;
D O I
10.1016/S0378-1119(96)00810-4
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec(-) and Rec(+) by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.
引用
收藏
页码:215 / 220
页数:6
相关论文
共 22 条
[1]   TRANSFORMATION-DEFICIENT MUTANTS OF PILIATED NEISSERIA-GONORRHOEAE [J].
BISWAS, GD ;
LACKS, SA ;
SPARLING, PF .
JOURNAL OF BACTERIOLOGY, 1989, 171 (02) :657-664
[2]   TRANSCRIPTIONAL CONTROL OF GONOCOCCAL PILE EXPRESSION - INVOLVEMENT OF AN ALTERNATE SIGMA-FACTOR [J].
BOYLEVAVRA, S ;
SO, M ;
SEIFERT, HS .
GENE, 1993, 137 (02) :233-236
[3]   SHUTTLE MUTAGENESIS - 2 MINI-TRANSPOSONS FOR GENE-MAPPING AND FOR LACZ TRANSCRIPTIONAL FUSIONS IN NEISSERIA-GONORRHOEAE [J].
BOYLEVAVRA, S ;
SEIFERT, HS .
GENE, 1993, 129 (01) :51-57
[4]  
BOYLEVAVRA S, 1996, MICROBIOLOGY, V142, P2838
[5]   PHYSICAL ANALYSIS OF THE CONJUGATIVE SHUTTLE TRANSPOSON-TN1545 [J].
CAILLAUD, F ;
CARLIER, C ;
COURVALIN, P .
PLASMID, 1987, 17 (01) :58-60
[6]   SPECIES-SPECIFIC UPTAKE OF DNA BY GONOCOCCI IS MEDIATED BY A 10-BASE-PAIR SEQUENCE [J].
ELKINS, C ;
THOMAS, CE ;
SEIFERT, HS ;
SPARLING, PF .
JOURNAL OF BACTERIOLOGY, 1991, 173 (12) :3911-3913
[7]   CHARACTERIZATION OF THE PILF-PILD PILUS-ASSEMBLY LOCUS OF NEISSERIA-GONORRHOEAE [J].
FREITAG, NE ;
SEIFERT, HS ;
KOOMEY, M .
MOLECULAR MICROBIOLOGY, 1995, 16 (03) :575-586
[8]   NUCLEOTIDE-SEQUENCE AND EXPRESSION IN ESCHERICHIA-COLI OF THE RECA GENE OF NEISSERIA-GONORRHOEAE [J].
FYFE, JAM ;
DAVIES, JK .
GENE, 1990, 93 (01) :151-156
[9]   REASSORTMENT OF PILIN GENES IN NEISSERIA-GONORRHOEAE OCCURS BY 2 DISTINCT MECHANISMS [J].
GIBBS, CP ;
REIMANN, BY ;
SCHULTZ, E ;
KAUFMANN, A ;
HAAS, R ;
MEYER, TF .
NATURE, 1989, 338 (6217) :651-652
[10]   IDENTIFICATION AND ARRANGEMENT OF THE DNA-SEQUENCE RECOGNIZED IN SPECIFIC TRANSFORMATION OF NEISSERIA-GONORRHOEAE [J].
GOODMAN, SD ;
SCOCCA, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6982-6986