Insertionally inactivated and inducible recA alleles for use in Neisseria

被引：86

作者：

Seifert, HS

机构：

[1] NW University Medical School, Dept. of Microbiology-Immunology, Mailcode W213, Searle 6-467, Chicago, IL 60611

来源：

GENE | 1997年 / 188卷 / 02期

关键词：

DNA recombination; lac operon; DNA transformation; Pilin variation; DNA repair;

D O I：

10.1016/S0378-1119(96)00810-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two classes of recA mutations have been constructed for use in Neisseria gonorrhoeae: three insertionally inactivated ('knockout') mutations and three LacI-regulatable constructs that can be shifted between Rec(-) and Rec(+) by the removal or addition of IPTG. The effects of regulating recA expression on the processes of DNA transformation, DNA repair and pilin-phase variation are described. These regulatable cassettes can also be used to control the expression of any chromosomal gene.

引用

页码：215 / 220

页数：6

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