Use of monoclonal antibodies for weak affinity chromatography

被引：32

作者：

Ohlson, S ^{[1
]}

Bergstrom, M ^{[1
]}

Pahlsson, P ^{[1
]}

Lundblad, A ^{[1
]}

机构：

[1] LINKOPING UNIV HOSP,DEPT CLIN CHEM,S-58185 LINKOPING,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 758卷 / 02期

关键词：

affinity chromatography; stationary phases; LC; monoclonal antibodies; antigens; carbohydrates;

D O I：

10.1016/S0021-9673(96)00733-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Weak monoclonal antibodies were used as ligands in a high-performance liquid affinity chromatography system. Isocratic weak affinity chromatography was achieved when similar carbohydrate antigens were separated according to their weak binding to the immobilized monoclonal antibody. These chromatographic systems were studied in detail in terms of affinity, specificity and efficiency. The influence of the physico-chemical factors of temperature, pH, ionic strength and organic solvents was also evaluated. The issue of specificity was specially considered, as non-specific interactions are prevalent and usually of a weak affinity nature. This study clearly demonstrates the potential to use weak affinity biological interactions as the basis of chromatographic analysis and separation.

引用

页码：199 / 208

页数：10

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[11] Isolation of antigens and antibodies by affinity chromatography [J].

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