The structure and spatio-temporal distribution of the Archaea in a horizontal subsurface flow constructed wetland

被引：18

作者：

Bouali, Moez ^{[1
,2
,3
]}

Zrafi-Nouira, Ines ^{[1
]}

Bakhrouf, Amina ^{[1
]}

Le Paslier, Denis ^{[3
]}

Chaussonnerie, Sebastien ^{[3
]}

Ammar, Emna ^{[2
]}

Sghir, Abdelghani ^{[3
,4
]}

机构：

[1] Univ Monastir, Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia

[2] Univ Sfax, ENIS, UR Etud & Gest Environm Urbains & Cotiers, Sfax 3038, Tunisia

[3] Genoscope, CNRS, CEA, Inst Genom,UMR 8030, F-91057 Evry, France

[4] Univ Evry Val dEssonne, F-91025 Evry, France

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2012年 / 435卷

关键词：

Wastewater treatment; Horizontal subsurface flow constructed wetland; 16S rRNA genes; Archaeal diversity; Thaumarchaeota; AMMONIA-OXIDIZING ARCHAEA; WASTE-WATER TREATMENT; BACTERIA; DIVERSITY; COMMUNITY; THAUMARCHAEOTA; REMOVAL; SLUDGE; PLANTS; AMOA;

D O I：

10.1016/j.scitotenv.2012.07.047

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, archaeal community structure and temporal dynamics were monitored, using 16S rRNA clone libraries construction from a horizontal subsurface flow constructed wetland. Phylogenetic assignation of 1026 16S rRNA gene sequences shows that 96.2% of the total operational taxonomic units (OTUs) were affiliated with Thaumarchaeota, a newly proposed archaeal phylum and 3.7% with unclassified Archaea. Among the total sequences, 42% and 40.2% were affiliated with Candidatus Nitrososphaera and unclassified Nitrosopumilus respectively with more than 99% similarity. Results suggest that several dominant and active nitrifiers may benefit from the micro-aerobic conditions around the reed roots to perform ammonia oxidation. The archaeal diversity detected in the rhizosphere zone is clearly different from that detected in the bottom basin. This engineered habitat revealed the reed root and the water composition effects on the archaeal diversity. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：465 / 471

页数：7

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