Physicochemical characterization of Pseudomonas stutzeri UFV5 and analysis of its transcriptome under heterotrophic nitrification/aerobic denitrification pathway induction condition

被引：31
作者：
Fidelis Silva, Livia Carneiro [1 ]
Lima, Helena Santiago [1 ]
de Oliveira Mendes, Tiago Antonio [2 ]
Sartoratto, Adilson [3 ]
Sousa, Maira Paula [4 ]
de Souza, Rodrigo Suhett [4 ]
de Paula, Sergio Oliveira [5 ]
de Oliveira, Valeria Maia [3 ]
Silva, Cynthia Canedo [1 ]
机构：
[1] Univ Fed Vicosa, Dept Microbiol, BR-36570900 Vicosa, MG, Brazil
[2] Univ Fed Vicosa, Dept Biochem, BR-36570900 Vicosa, MG, Brazil
[3] Univ Estadual Campinas, Pluridisciplinary Ctr Chem Biol & Agr Res, BR-13083970 Campinas, SP, Brazil
[4] Petrobras SA, Petrobras Res & Dev Ctr CENPES, BR-21941915 Rio De Janeiro, Brazil
[5] Univ Fed Vicosa, Dept Gen Biol, BR-36570900 Vicosa, MG, Brazil
来源：
SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期
关键词：
AEROBIC DENITRIFYING BACTERIUM; POTENTIAL APPLICATION; NITROGEN REMOVAL; AMMONIUM REMOVAL; IDENTIFICATION; PURIFICATION; OXIDATION; SEQUENCE;
D O I：
10.1038/s41598-020-59279-7
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Biological ammonium removal via heterotrophic nitrification/aerobic denitrification (HN/AD) presents several advantages in relation to conventional removal processes, but little is known about the microorganisms and metabolic pathways involved in this process. In this study, Pseudomonas stutzeri UFV5 was isolated from an activated sludge sample from oil wastewater treatment station and its ammonium removal via HN/AD was investigated by physicochemical and molecular approaches to better understand this process and optimize the biological ammonium removal in wastewater treatment plants. Results showed that P. stutzeri UFV5 removed all the ammonium in 48-72hours using pyruvate, acetate, citrate or sodium succinate as carbon sources, C/N ratios 6, 8, 10 and 12, 3-6% salinities, pH 7-9 and temperatures of 20-40 degrees C. Comparative genomics and PCR revealed that genes encoding the enzymes involved in anaerobic denitrification process are present in P. stutzeri genome, but no gene that encodes enzymes involved in autotrophic nitrification was found. Furthermore, transcriptomics showed that none of the known enzymes of autotrophic nitrification and anaerobic denitrification had their expression differentiated and an upregulation of the biosynthesis machinery and protein translation was observed, besides several genes with unknown function, indicating a non-conventional mechanism involved in HN/AD process.
引用
收藏
页数：13
相关论文
共 52 条
[41]  
Team R. C., 2014, R LANG ENV STAT COMP
[42]   The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools [J].
Thompson, JD ;
Gibson, TJ ;
Plewniak, F ;
Jeanmougin, F ;
Higgins, DG .
NUCLEIC ACIDS RESEARCH, 1997, 25 (24) :4876-4882
[43]   Validation of the correct start codon of norX/nxrX and universality of the norAXB/nxrAXB gene cluster in Nitrobacter species [J].
Vanparys, B. ;
Bodelier, P. ;
De Vos, P. .
CURRENT MICROBIOLOGY, 2006, 53 (03) :255-257
[44]   Aerobic denitrification by novel isolated strain using NO2--N as nitrogen source [J].
Wan, Chunli ;
Yang, Xue ;
Lee, Duu-Jong ;
Du, Maoan ;
Wan, Fang ;
Chen, Chuan .
BIORESOURCE TECHNOLOGY, 2011, 102 (15) :7244-7248
[45]   Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species [J].
Wehrfritz, JM ;
Carter, JP ;
Spiro, S ;
Richardson, DJ .
ARCHIVES OF MICROBIOLOGY, 1996, 166 (06) :421-424
[46]   PURIFICATION OF HYDROXYLAMINE OXIDASE FROM THIOSPHAERA-PANTOTROPHA - IDENTIFICATION OF ELECTRON ACCEPTERS THAT COUPLE HETEROTROPHIC NITRIFICATION TO AEROBIC DENITRIFICATION [J].
WEHRFRITZ, JM ;
REILLY, A ;
SPIRO, S ;
RICHARDSON, DJ .
FEBS LETTERS, 1993, 335 (02) :246-250
[47]   Nitrogen Removal Characteristics of Pseudomonas putida Y-9 Capable of Heterotrophic Nitrification and Aerobic Denitrification at Low Temperature [J].
Xu, Yi ;
He, Tengxia ;
Li, Zhenlun ;
Ye, Qing ;
Chen, Yanli ;
Xie, Enyu ;
Zhang, Xue .
BIOMED RESEARCH INTERNATIONAL, 2017, 2017
[48]   Nitrogen removal characteristics of a heterotrophic nitrifier Acinetobacter junii YB and its potential application for the treatment of high-strength nitrogenous wastewater [J].
Yang, Lei ;
Ren, Yong-Xiang ;
Liang, Xian ;
Zhao, Si-Qi ;
Wang, Jun-ping ;
Xia, Zhi-Hong .
BIORESOURCE TECHNOLOGY, 2015, 193 :227-233
[49]   Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium, Acinetobacter sp HA2 [J].
Yao, Shuo ;
Ni, Jinren ;
Ma, Tao ;
Li, Can .
BIORESOURCE TECHNOLOGY, 2013, 139 :80-86
[50]   Paracoccus versutus KS293 adaptation to aerobic and anaerobic denitrification: Insights from nitrogen removal, functional gene abundance, and proteomic profiling analysis [J].
Zhang, Haihan ;
Zhao, Zhenfang ;
Chen, Shengnan ;
Kang, Pengliang ;
Wang, Yue ;
Feng, Ji ;
Jia, Jingyu ;
Yan, Miaomiao ;
Wang, Yan ;
Xu, Lei .
BIORESOURCE TECHNOLOGY, 2018, 260 :321-328
123456