Immunological Evaluation of Co-Assembling a Lipidated Peptide Antigen and Lipophilic Adjuvants: Self-Adjuvanting Anti-Breast-Cancer Vaccine Candidates

被引：23

作者：

Aiga, Taku ^{[1
]}

Manabe, Yoshiyuki ^{[1
,2
]}

Ito, Keita ^{[1
,2
]}

Chang, Tsung-Che ^{[1
]}

Kabayama, Kazuya ^{[1
,2
]}

Ohshima, Shino ^{[3
]}

Kametani, Yoshie ^{[3
]}

Miura, Ayane ^{[1
]}

Furukawa, Hiroto ^{[4
]}

Inaba, Hiroshi ^{[4
]}

Matsuura, Kazunori ^{[4
]}

Fukase, Koichi ^{[1
,2
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Chem, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan

[2] Osaka Univ, Project Res Ctr Fundamental Sci, Grad Sch Sci, Core Med & Sci Collaborat Res & Educ, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan

[3] Tokai Univ, Sch Med, Isehara, Kanagawa 2591193, Japan

[4] Tottori Univ, Ctr Res Green Sustainable Chem, Grad Sch Engn, Dept Chem & Biotechnol, 4-101 Koyama Minami, Tottori 6808552, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2020年 / 59卷 / 40期

关键词：

adjuvants; antigens; cancer; peptides; self-assembly; ROBUST IMMUNE-RESPONSES; CLINICAL-TRIAL; ALPHA-GALACTOSYLCERAMIDES; LIPOPEPTIDE ADJUVANTS; MITOGENIC PRINCIPLE; CONJUGATE VACCINES; CELL-ACTIVATION; RECOGNITION; AGONIST; MUC1;

D O I：

10.1002/anie.202007999

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Co-assembling vaccines composed of a lipidated HER2-derived antigenic CH401 peptide and either a lipophilic adjuvant, Pam(3)CSK(4), alpha-GalCer, or lipid A 506, were evaluated as breast cancer vaccine candidates. This vaccine design was aimed to inherit both antigen multivalency and antigen-specific immunostimulation properties, observed in reported self-adjuvanting vaccine candidates, by using self-assembly and adjuvant-conjugated antigens. Under vaccination concentrations, respective lipophilic adjuvants underwent co-assembly with lipidated CH401, which boosted the anti-CH401 IgG and IgM production. In particular, alpha-GalCer was responsible for the most significant immune activation. Therefore, the newly developed vaccine design enabled the optimization of adjuvants against the antigenic CH401 peptide in a simple preparatory manner. Overall, the co-assembling vaccine design opens the door for efficient and practical self-adjuvanting vaccine development.

引用

页码：17705 / 17711

页数：7

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