Expression of CD24 on CD19-CD79a+ early B-cell progenitors in human bone marrow

被引：15
作者：
Israel, E
Kapelushnik, J
Yermiahu, T
Levi, I
Yaniv, I
Shpilberg, O
Shubinsky, G [1 ]
机构：
[1] Soroka Univ, Med Ctr, Flow Cytometry Unit, Beer Sheva, Israel
[2] Soroka Univ, Med Ctr, Inst Hematol, Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Fac Hlth Sci, Dept Immunol, IL-84105 Beer Sheva, Israel
[4] Schneider Childrens Med Ctr Israel, Dept Hematol Oncol, Petah Tiqwa, Israel
来源：
CELLULAR IMMUNOLOGY | 2005年 / 236卷 / 1-2期
关键词：
early B-cell progenitors; CD24; human bone marrow; B lymphocyte maturation;
D O I：
10.1016/j.cellimm.2005.08.026
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
CD24 is a surface marker expressed in immature and mature B cells and involved in cellular adhesion and apoptosis. There are no data, which delineate the stage in early development of human B cells, which marks the expression of CD24. We studied lymphopoiesis in normal pediatric bone marrow (BM) and found that 1.5 +/- 0.2% of WBC were CD24(+) lymphocytes which did not express CD19. A significant fraction of these cells expressed low levels of CD45 (CD19(-)CD24(+)CD45(low) cells). Small numbers of CD19(-)CD24(+)CD45(low) cells were found in the regenerating BM of children with acute lymphoblastic leukemia after the completion of chemotherapy and in normal adult BM. Flow cytometric analyses have shown that CD19(-)CD24(+)CD45(low) lymphocytes express CD10, CD34, CD79a, CD79a (VpreB), and TdT markers, i.e., displayed antigenic properties of early B-cell progenitors. Our data indicate that CD19(-) early B-cell progenitors in human BM express CD24, and that the expression of CD24 in human B-cell development precedes the expression of CD19. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：171 / 178
页数：8
相关论文
共 40 条
[1]   Ig D-H gene segment transcription and rearrangement before surface expression of the pan-B cell marker CD19 in normal human bone marrow [J].
Bertrand, FE ;
Billips, LG ;
Burrows, PD ;
Gartland, GL ;
Kubagawa, H ;
Schroeder, HW .
BLOOD, 1997, 90 (02) :736-744
[2]  
BOFILL M, 1985, J IMMUNOL, V134, P1531
[3]   Early onset of immunoglobulin heavy chain gene rearrangements in normal human bone marrow CD34(+) cells [J].
Davi, F ;
Faili, A ;
Gritti, C ;
Blanc, C ;
Laurent, C ;
Sutton, L ;
Schmitt, C ;
MerleBeral, H .
BLOOD, 1997, 90 (10) :4014-4021
[4]  
DUPERRAY C, 1990, J IMMUNOL, V145, P3678
[5]   Four-color flow cytometric investigation of terminal deoxynucleotidyl transferase-positive lymphoid precursors in pediatric bone marrow:: CD79a expression precedes CD19 in early B-cell ontogeny [J].
Dworzak, MN ;
Fritsch, G ;
Fröschl, G ;
Printz, D ;
Gadner, H .
BLOOD, 1998, 92 (09) :3203-3209
[6]   Multiparameter phenotype mapping of normal and post-chemotherapy B lymphopoiesis in pediatric bone marrow [J].
Dworzak, MN ;
Fritsch, G ;
Fleischer, C ;
Printz, D ;
Froschl, G ;
Buchinger, P ;
Mann, G ;
Gadner, H .
LEUKEMIA, 1997, 11 (08) :1266-1273
[7]   Assessment of CD24 expression on bone marrow neutrophilic granulocytes: CD24 is a marker for the myelocytic stage of development [J].
Elghetany, MT ;
Patel, J .
AMERICAN JOURNAL OF HEMATOLOGY, 2002, 71 (04) :348-349
[8]  
Fluckiger AC, 1998, BLOOD, V92, P4509
[9]   HUMAN T-CELLS, B-CELLS, NATURAL-KILLER, AND DENDRITIC CELLS ARISE FROM A COMMON BONE-MARROW PROGENITOR-CELL SUBSET [J].
GALY, A ;
TRAVIS, M ;
CEN, DZ ;
CHEN, B .
IMMUNITY, 1995, 3 (04) :459-473
[10]   Ordering of human bone marrow B lymphocyte precursors by single-cell polymerase chain reaction analyses of the rearrangement status of the immunoglobulin H and L chain gene loci [J].
Ghia, P ;
tenBoekel, E ;
Sanz, E ;
delaHera, A ;
Rolink, A ;
Melchers, F .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (06) :2217-2229
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