Ammonium-dependent hydrogen peroxide production by mitochondria

被引：19
作者：
Grivennikova, Vera G. [1 ]
Cecchini, Gary [2 ,4 ]
Vinogradov, Andrei D. [1 ,3 ]
机构：
[1] Moscow MV Lomonosov State Univ, Sch Biol, Dept Biochem, Moscow 119992, Russia
[2] VA Med Ctr, Div Mol Biol, San Francisco, CA 94121 USA
[3] Moscow MV Lomonosov State Univ, Mitoengn Ctr, Moscow 119992, Russia
[4] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
来源：
FEBS LETTERS | 2008年 / 582卷 / 18期
关键词：
hydrogen peroxide; mitochondria; complex I; ammonium;
D O I：
10.1016/j.febslet.2008.06.054
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly ( similar to 10fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide ( hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH: oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码：2719 / 2724
页数：6
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