Ammonium-dependent hydrogen peroxide production by mitochondria

被引：19
作者：
Grivennikova, Vera G. [1 ]
Cecchini, Gary [2 ,4 ]
Vinogradov, Andrei D. [1 ,3 ]
机构：
[1] Moscow MV Lomonosov State Univ, Sch Biol, Dept Biochem, Moscow 119992, Russia
[2] VA Med Ctr, Div Mol Biol, San Francisco, CA 94121 USA
[3] Moscow MV Lomonosov State Univ, Mitoengn Ctr, Moscow 119992, Russia
[4] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
来源：
FEBS LETTERS | 2008年 / 582卷 / 18期
关键词：
hydrogen peroxide; mitochondria; complex I; ammonium;
D O I：
10.1016/j.febslet.2008.06.054
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly ( similar to 10fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide ( hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH: oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
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页码：2719 / 2724
页数：6
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