Plectasin has antibacterial activity and no affect on cell viability or IL-8 production

被引:50
作者
Hara, Shintaro [1 ]
Mukae, Hiroshi [1 ]
Sakamoto, Noriho [1 ]
Ishimoto, Hiroshi [1 ]
Amenomori, Misato [1 ]
Fujita, Hanako [1 ]
Ishimatsu, Yuji [1 ]
Yanagihara, Katsunori [2 ]
Kohno, Shigeru [1 ]
机构
[1] Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan
[2] Nagasaki Univ, Sch Med, Dept Lab Med, Nagasaki 8528501, Japan
关键词
plectasin; human defensins; antibacterial activity; cytokine induction; cytotoxicity; defensin; human neutrophil peptide-1; interleukin-8; induction; A549; cell; normal human bronchial epithelial cell; normal human lung fibroblast;
D O I
10.1016/j.bbrc.2008.07.093
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Animals and plants express endogenous peptide antibiotics called defensins. Defensins show broad-spectrum antimicrobial activity, even against bacteria that have resistance to conventional antibiotics, which has made them viable candidates for new antibiotics. However, human defensins have failed to reach the market because of their cytotoxic effects and non-antimicrobial bioactivities. Plectasin is a defensin that has shown promise but has not had its potentially negative effects clarified. To address this issue, we examined plectasin's cytotoxicity in human cells using an AlamarBlue reduction assay, its interleukin (IL)-8-inducing capacity using real-time PCR and ELISA, and measured its MIC against bacteria. We confirmed that plectasin has specific antibacterial activity against Streptococcus pneumoniae. Plectasin showed no cytotoxicity to A549 cells, normal human bronchial epithelial cells, or lung fibroblasts, and it did not induce IL-8 transcription or production in A549 cells. Our results suggest that plectasin could be an inoffensive alternative antibiotic for clinical application. (c) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:709 / 713
页数:5
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