Multi-walled Carbon Nanotubes Penetrate into Plant Cells and Affect the Growth of Onobrychis arenaria Seedlings

被引:17
作者
Smirnova, E. A. [1 ]
Gusev, A. A.
Zaitseva, O. N.
Lazareva, E. M. [1 ]
Onishchenko, G. E. [1 ]
Kuznetsova, E. V. [2 ]
Tkachev, A. G.
Feofanov, A. V. [1 ,3 ]
Kirpichnikov, M. P. [1 ,3 ]
机构
[1] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 117234, Russia
[2] Russian Acad Sci, Siberian Branch, Siberian Inst Plant Physiol & Biochem, Moscow 117901, Russia
[3] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117901, Russia
来源
ACTA NATURAE | 2011年 / 3卷 / 01期
关键词
multi-walled carbon nanotubes; light microscopy; electron microscopy; electron diffraction pattern; O. arenaria seedlings; ENGINEERED NANOPARTICLES; NANOMATERIALS; ECOTOXICITY;
D O I
10.32607/20758251-2011-3-1-99-106
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Engineered nanoparticles (ENPs) are now being used in many sectors of industry; however, the impact of ENPs on the environment still requires further study, since their use, recycling, and accidental spill can result in the accumulation of nanoparticles in the atmosphere, soil, and water. Plants are an integral part of ecosystems; hence their interaction with ENPs is inevitable. It is important to understand the consequences of this interaction and assess its potential effects. The present research is focused on studying the effects of the industrial material Taunit, containing multi-walled carbon nanotubes (MWNTs), on plants, and testing of its ability to penetrate into plant cells and tissues. Taunit has been found to stimulate the growth of roots and stems and cause an increase in peroxidase activity in Onobrychis arenaria seedlings. Peroxidase activity increases with decreasing concentration of Taunit from 1,000 to 100 mg/l. MWNTs from Taunit were detected in the cells and tissues of seedling roots and leaves, implying the ability of MWNTs to penetrate into roots and accumulate there, as well as their ability to be transported into seedling leaves. Thus, the changes in the physiological parameters of plants are associated not only with MWNT adsorption on the root surface, as previously believed, but also with their penetration, uptake and accumulation in the plant cells and tissues.
引用
收藏
页码:99 / 106
页数:8
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