Differential long noncoding RNAs expression in cancer-associated fibroblasts of non-small-cell lung cancer

被引：11

作者：

Teng, Cuifang ^{[1
]}

Huang, Guolin ^{[1
]}

Luo, Yanmei ^{[1
]}

Pan, Yingxing ^{[1
]}

Wang, Hanlin ^{[2
]}

Liao, Xiaoli ^{[3
]}

Li, Yongqiang ^{[3
]}

Yang, Jie ^{[1
]}

机构：

[1] Guangxi Med Univ, Sch Pharm, Dept Pharmacol, Nanning 530021, Guangxi, Peoples R China

[2] Guangxi Med Univ, Dept Internal Med, Affiliated Hosp 1, Nanning 530021, Guangxi, Peoples R China

[3] Guangxi Med Univ, Dept Chemotherapy, Affiliated Canc Hosp, Nanning 530021, Guangxi, Peoples R China

来源：

PHARMACOGENOMICS | 2019年 / 20卷 / 03期

基金：

中国国家自然科学基金;

关键词：

cancer-associated fibroblasts; immune system; long noncoding RNAs; non-small-cell lung cancer; PROLIFERATION; SUPPRESSION; RESISTANCE;

D O I：

10.2217/pgs-2018-0102

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Aim: The aim of this study was to investigate the role of long noncoding RNAs (lncRNAs) profiles in cancer-associated fibroblasts (CAFs) during non-small-cell lung cancer (NSCLC) progression. Materials & methods: Differentially expressed lncRNAs and mRNAs were detected by lncRNA microarray between three patient-paired CAFs and the adjacent normal fibroblasts, which were obtained from tumoral and nontumoral portions of surgically resected lung tissue from three primary NSCLCs. Bioinformatic analyses including gene ontology and pathway analysis were applied to these differentially expressed mRNAs. The qRT-PCR was conducted to identify the change of selected lncRNAs that might be involved in contribution of CAFs toward NSCLC. Results:A total of766 lncRNAs and 750 mRNAs abnormally expressed in CAFs (fold-change >2, p<0.05). Bioinformatic analyses indicated that these mRNAs are associated with immune function. The qPCR results were consistent with microarray data. Conclusion: The lncRNAs profiles of CAFs may provide promising targets for further research on immune regulation during NSCLC process.

引用

页码：143 / 153

页数：11

共 50 条

[41] Crosstalk between colorectal cancer cells and cancer-associated fibroblasts in the tumor microenvironment mediated by exosomal noncoding RNAs [J].

Sun, Shichen ;

Zhang, Yanyu ;

Li, Yubing ;

Wei, Linlin .

FRONTIERS IN IMMUNOLOGY, 2023, 14

[42] Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-Small Cell Lung Cancer [J].

Nowinska, Katarzyna ;

Jablonska, Karolina ;

Pawelczyk, Konrad ;

Piotrowska, Aleksandra ;

Partynska, Aleksandra ;

Gomulkiewicz, Agnieszka ;

Ciesielska, Urszula ;

Katnik, Ewa ;

Grzegrzolka, Jedrzej ;

Glatzel-Plucinska, Natalia ;

Ratajczak-Wielgomas, Katarzyna ;

Podhorska-Okolow, Marzenna ;

Dziegiel, Piotr .

CANCERS, 2019, 11 (10)

[43] Increased Expression and Activation of FAK in Small-Cell Lung Cancer Compared to Non-Small-Cell Lung Cancer [J].

Nana, Frank Aboubakar ;

Hoton, Delphine ;

Ambroise, Jerome ;

Lecocq, Marylene ;

Vanderputten, Marie ;

Sibille, Yves ;

Vanaudenaerde, Bart ;

Pilette, Charles ;

Bouzin, Caroline ;

Ocak, Sebahat .

CANCERS, 2019, 11 (10)

[44] The promising role of noncoding RNAs in cancer-associated fibroblasts: an overview of current status and future perspectives [J].

Zengli Fang ;

Jin Xu ;

Bo Zhang ;

Wei Wang ;

Jiang Liu ;

Chen Liang ;

Jie Hua ;

Qingcai Meng ;

Xianjun Yu ;

Si Shi .

Journal of Hematology & Oncology, 13

[45] The promising role of noncoding RNAs in cancer-associated fibroblasts: an overview of current status and future perspectives [J].

Fang, Zengli ;

Xu, Jin ;

Zhang, Bo ;

Wang, Wei ;

Liu, Jiang ;

Liang, Chen ;

Hua, Jie ;

Meng, Qingcai ;

Yu, Xianjun ;

Shi, Si .

JOURNAL OF HEMATOLOGY & ONCOLOGY, 2020, 13 (01)

[46] Comprehensive landscape and future perspective of long noncoding RNAs in non-small cell lung cancer: it takes a village [J].

Ao, Yong-Qiang ;

Gao, Jian ;

Jiang, Jia-Hao ;

Wang, Hai-Kun ;

Wang, Shuai ;

Ding, Jian-Yong .

MOLECULAR THERAPY, 2023, 31 (12) :3389-3413

[47] Comprehensive analysis of differentially expressed long non-coding RNAs in non-small cell lung cancer [J].

Zhou, Wenyong ;

Liu, Tao ;

Saren, Gaowa ;

Liao, Li ;

Fang, Wentao ;

Zhao, Heng .

ONCOLOGY LETTERS, 2019, 18 (02) :1145-1156

[48] The expression of β-catenin in non-small-cell lung cancer:: a clinicopathological study [J].

Retera, JMAM ;

Leers, MPG ;

Sulzer, MA ;

Theunissen, PHMH .

JOURNAL OF CLINICAL PATHOLOGY, 1998, 51 (12) :891-894

[49] Immunotherapy for non-small-cell lung cancer [J].

Thomas, Anish ;

Jakopovic, Marko .

EXPERT OPINION ON BIOLOGICAL THERAPY, 2014, 14 (08) :1061-1064

[50] Staging of Non-Small-Cell Lung Cancer [J].

Akhurst, Tim .

PET CLINICS, 2018, 13 (01) :1-+

← 1 2 3 4 5 →