Microarray studies of factor VIIa-activated cancer cells

被引:11
作者
Petersen, Lars Christian [1 ]
机构
[1] Novo Nordisk, Haemostasis Biol, DK-2760 Malov, Denmark
关键词
factor VIIa; protease activated receptors; signaling; microarray;
D O I
10.1016/S0049-3848(08)70011-2
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Factor VIIa (FVIIa)-induced signal transduction is strongly dependent on cellular surface expression of Tissue Factor (TF) and Protease Activated Receptors (PARs). FVIIa signals primarily through PAR2. This contrasts to thrombin which signals primarily via PAR1 and does so without the assistance of a co-receptor, but by binding to an exosite on PAR1. Various TF: FVII-mediated cellular activities are now well documented and have indicated possible links to inflammation, atherosclerosis, angiogenesis, tissue repair, tumor growth and metastasis. Further knowledge about cellular responses induced by coagulation factors has been obtained by gene-expression profiling of MDA-MB-231 cells stimulated with FVIIa or alternatively with PAR1 or PAR2 agonist peptides. These studies and qPCR measurements of the transcription of selected genes in these and other carcinoma cell lines have provided new information about gene expression induced by PAR activation, the gene repertoire induced by TF:FVIIa via PAR2, and how it differs from that induced via PAR1 by thrombin. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:S11 / S13
页数:3
相关论文
共 22 条
[1]   Transcriptional program induced by factor VIIa-tissue factor, PAR1 and PAR2 in MDA-MB-231 cells [J].
Albrektsen, T. ;
Sorensen, B. B. ;
Hjorto, G. M. ;
Fleckner, J. ;
Rao, L. V. M. ;
Petersen, L. C. .
JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2007, 5 (08) :1588-1597
[2]   Inflammation and cancer: back to Virchow? [J].
Balkwill, F ;
Mantovani, A .
LANCET, 2001, 357 (9255) :539-545
[3]  
Belperio JA, 2000, J LEUKOCYTE BIOL, V68, P1
[4]   Signaling of the tissue factor coagulation pathway in angiogenesis and cancer [J].
Belting, M ;
Ahamed, J ;
Ruf, W .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (08) :1545-1550
[5]   Regulation of angiogenesis by tissue factor cytoplasmic domain signaling [J].
Belting, M ;
Dorrell, MI ;
Sandgren, S ;
Aguilar, E ;
Ahamed, J ;
Dorfleutner, A ;
Carmeliet, P ;
Mueller, BM ;
Friedlander, M ;
Ruf, W .
NATURE MEDICINE, 2004, 10 (05) :502-509
[6]   Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa [J].
Camerer, E ;
Huang, W ;
Coughlin, SR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) :5255-5260
[7]   Binding of Factor VIIa to tissue factor on keratinocytes induces gene expression [J].
Camerer, E ;
Gjernes, E ;
Wiiger, M ;
Pringle, S ;
Prydz, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (09) :6580-6585
[8]   Thrombin signalling and protease-activated receptors [J].
Coughlin, SR .
NATURE, 2000, 407 (6801) :258-264
[9]   Upregulation of interleukin 8 by oxygen-deprived cells in glioblastoma suggests a role in leukocyte activation, chemotaxis, and angiogenesis [J].
Desbaillets, I ;
Diserens, AC ;
deTribolet, N ;
Hamou, MF ;
Van Meir, EG .
JOURNAL OF EXPERIMENTAL MEDICINE, 1997, 186 (08) :1201-1212
[10]  
DVORAK HF, 1986, NEW ENGL J MED, V315, P1650