The effect of hematocrit on bioanalysis of DBS: results from the EBF DBS-microsampling consortium

被引:106
作者
de Vries, Ronald [1 ]
Barfield, Matthew [2 ]
van de Merbel, Nico [3 ]
Schmid, Bernhard [4 ]
Siethoff, Christoph [5 ]
Ortiz, Jordi [6 ]
Verheij, Elwin [7 ]
van Baar, Ben [8 ]
Cobb, Zoe [9 ]
White, Steve [2 ]
Timmerman, Philip [1 ]
机构
[1] Janssen R&D NV, B-2340 Beerse, Belgium
[2] GlaxoSmithKline, Ware, Herts, England
[3] PRA, Assen, Netherlands
[4] Nuvisan GmbH, Neu Ulm, Germany
[5] Swiss BioQuant, Reinach, Switzerland
[6] Ferrer Int SA, Esplugas de Llobregat, Spain
[7] TNO, NL-3700 AJ Zeist, Netherlands
[8] QPS Netherlands BV, Groningen, Netherlands
[9] LGC Grp, Quotient Bio Analyt Sci, Fordham, Cambs, England
关键词
DRIED BLOOD SPOTS; QUANTITATIVE BIOANALYSIS; ASSAY BIAS;
D O I
10.4155/bio.13.170
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Background: The European Bioanalysis Forum dried blood spots (DBS)/microsampling consortium is reporting back from the experiments they performed on further documenting the potential hurdles of the DBS technology. This paper is focused on the impact of hematocrit changes on DBS analyses. Results: The hematocrit can have an effect on the size of the blood spot, on spot homogeneity and on extraction recovery in a compound-dependent manner. The extraction recovery can change upon aging in an hematocrit-dependent way. Different card materials can give different outcomes. Conclusions: The results from the conducted experiments show that the issues of DBS in regulated bioanalysis are real and that the technology will need improvements to be ready for use as a general tool for regulated bioanalysis. © 2013 Future Science Ltd.
引用
收藏
页码:2147 / 2160
页数:14
相关论文
共 23 条
[1]  
[Anonymous], 2001, GUID IND BIOAN METH
[2]   Prediction of the Hematocrit of Dried Blood Spots via Potassium Measurement on a Routine Clinical Chemistry Analyzer [J].
Capiau, Sara ;
Stove, Veronique V. ;
Lambert, Willy E. ;
Stove, Christophe P. .
ANALYTICAL CHEMISTRY, 2013, 85 (01) :404-410
[3]  
Cobb Z, 2013, BIOANALYSIS, V5, P2161, DOI [10.4155/bio.13.171, 10.4155/BIO.13.171]
[4]   The effect of hematocrit on assay bias when using DBS samples for the quantitative bioanalysis of drugs [J].
Denniff, Philip ;
Spooner, Neil .
BIOANALYSIS, 2010, 2 (08) :1385-1395
[5]  
Dijksman J, C EUR BIOAN FOR 5 OP
[6]  
Dijksman J, 2012, BIOANALYSIS, V4, P633, DOI [10.4155/BIO.12.36, 10.4155/bio.12.36]
[7]   Hematocrit, volume expander, temperature, and shear rate effects on blood viscosity [J].
Eckmann, DM ;
Bowers, S ;
Stecker, M ;
Cheung, AT .
ANESTHESIA AND ANALGESIA, 2000, 91 (03) :539-545
[8]  
European Medicines Agency, 2011, 1922172009 EMEA CHMP
[9]  
Fan LM, 2012, BIOANALYSIS, V4, P345, DOI [10.4155/BIO.11.337, 10.4155/bio.11.337]
[10]  
GUTHRIE R, 1963, PEDIATRICS, V32, P338