Photophysics and photochemistry of zinc phthalocyanine/bovine serum albumin adducts

被引：48

作者：

Alarcon, Emilio ^{[1
]}

Edwards, Ana Maria ^{[1
]}

Garcia, Angelica M. ^{[1
]}

Munoz, Marcelo ^{[1
]}

Aspee, Alexis ^{[2
]}

Borsarelli, Claudio D. ^{[3
]}

Lissi, Eduardo A. ^{[2
]}

机构：

[1] Pontificia Univ Catolica Chile, Dept Quim Fis, Fac Quim, Santiago, Chile

[2] Univ Santiago Chile, Fac Quim & Biol, Dept Ciencias Ambiente, Santiago, Chile

[3] Univ Nacl Santiago Estero, Fac Agron & Agroind, Inst Quim, NOA INQUINOA CONICET, Santiago, Argentina

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2009年 / 8卷 / 02期

关键词：

PHOTODYNAMIC THERAPY; SILICON(IV) PHTHALOCYANINES; BINDING; PHOTOSENSITIZERS; FLUORESCENCE; OXIDATION; PROTEINS; DELIVERY;

D O I：

10.1039/b815726j

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc phthalocyanine (ZnPc) is a well known Type II (singlet oxygen mediated) hydrophobic photosensitizer with potential use in PDT. We have found that the presence of bovine serum albumin diminishes the aggregation degree of ZnPc in aqueous solution, indicating that albumins could be potentially useful carriers for this type of photosensitizer in PDT. In order to explore the photochemical and photophysical behavior of ZnPc associated to the protein, we have evaluated triplet excited state lifetime and yield, dye bleaching, oxygen consumption, formation of carbonyls and peroxides, and the spontaneous chemiluminiscence emitted after photolysis. The results show that dye association to BSA modifies the photophysics and photochemistry of ZnPC. In particular the decreased yield of long lived triplets suggests singlet state and/or static triplet quenching of the bound dye by the host protein.

引用

页码：255 / 263

页数：9

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