Serum soluble Fas ligand is a severity and mortality prognostic marker for COVID-19 patients

被引:16
|
作者
Saleki, Kiarash [1 ,2 ,3 ,4 ]
Shirzad, Moein [1 ,2 ,4 ]
Javanian, Mostafa [5 ]
Mohammadkhani, Sheyda [2 ]
Alijani, Mohammad Hossein [2 ]
Miri, Niloufarsadat [1 ]
Oladnabi, Morteza [6 ]
Azadmehr, Abbas [1 ,4 ,7 ]
机构
[1] Babol Univ Med Sci, Hlth Res Inst, Cellular & Mol Biol Res Ctr, Babol, Iran
[2] Babol Univ Med Sci, Student Res Comm, Babol, Iran
[3] Babol Univ Med Sci, USERN Off, Babol, Iran
[4] Natl Elite Fdn, Mazandaran Prov Branch, Mazandaran, Iran
[5] Babol Univ Med Sci, Hlth Res Inst, Infect Dis & Trop Med Res Ctr, Babol, Iran
[6] Golestan Univ Med Sci, Ischem Disorders Res Ctr, Gorgan, Iran
[7] Babol Univ Med Sci, Immunol Dept, Babol, Iran
来源
FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷
关键词
COVID-19; hyperinflammation; Fas; viral Immunology; immunoinformatics; AA FORCE-FIELD; EXPRESSION; APOPTOSIS; INJURY; ACTIVATION; VIRUS; GENE;
D O I
10.3389/fimmu.2022.947401
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Finding cytokine storm initiator factors associated with uncontrolled inflammatory immune response is necessary in COVID-19 patients. The aim was the identification of Fas/Fas Ligand (FasL) role in lung involvement and mortality of COVID-19 patients. In this case-control study, mild (outpatient), moderate (hospitalized), and severe (ICU) COVID-19 patients and healthy subjects were investigated. RNA isolated from PBMCs for cDNA synthesis and expression of mFas/mFasL mRNA was evaluated by RT-PCR. Serum sFas/sFasL protein by ELISA and severity of lung involvement by CT-scan were evaluated. Also, we docked Fas and FasL via Bioinformatics software (in silico) to predict the best-fit Fas/FasL complex and performed molecular dynamics simulation (MDS) in hyponatremia and fever (COVID-19 patients), and healthy conditions. mFasL expression was increased in moderate and severe COVID-19 patients compared to the control group. Moreover, mFas expression showed an inverse correlation with myalgia symptom in COVID-19 patients. Elevation of sFasL protein in serum was associated with reduced lung injury and mortality. Bioinformatics analysis confirmed that blood profile alterations of COVID-19 patients, such as fever and hyponatremia could affect Fas/FasL complex interactions. Our translational findings showed that decreased sFasL is associated with lung involvement; severity and mortality in COVID-19 patients. We think that sFasL is a mediator of neutrophilia and lymphopenia in COVID-19. However, additional investigation is suggested. This is the first report describing that the serum sFasL protein is a severity and mortality prognostic marker for the clinical management of COVID-19 patients.
引用
收藏
页数:20
相关论文
共 50 条
  • [1] Prognostic Value of Serum MICA Levels as a Marker of Severity in COVID-19 Patients
    Farzad, Faramarz
    Yaghoubi, Neda
    Jabbari-Azad, Farahzad
    Mahmoudi, Mahmoud
    Mohammadi, Mojgan
    IMMUNOLOGICAL INVESTIGATIONS, 2022, 51 (06) : 1856 - 1866
  • [2] Prognostic Significance of Soluble Fas and Soluble Fas Ligand in Serum of Patients with Complete Hydatidiform Moles
    Soni, Simmi
    Rath, Gayatri
    Deval, Ravi
    Salhan, Sudha
    Mishra, Ashwini Kumar
    Saxena, Sunita
    AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2011, 66 (03) : 230 - 236
  • [3] Prognostic significance of a combination of soluble Fas and soluble Fas ligand in the serum of patients with Ta bladder cancer
    Mizutani, Y
    Yoshida, O
    Ukimura, O
    Kawauchi, A
    Bonavida, B
    Miki, T
    CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2002, 17 (05) : 563 - 567
  • [4] Glucose variability is a marker for COVID-19 severity and mortality
    Abuhasira, Ran
    Grossman, Alon
    ARCHIVES OF ENDOCRINOLOGY METABOLISM, 2022, 66 (06): : 856 - 862
  • [5] Prognostic factors for predicting severity and mortality in hospitalized COVID-19 patients
    Aljohani, Fahad Dakilallah
    Khattab, Amin
    Elbadawy, Hossein M.
    Alhaddad, Aisha
    Alahmadey, Ziab
    Alahmadi, Yaser
    Eltahir, Heba M.
    Matar, Heba M. H.
    Wanas, Hanaa
    JOURNAL OF CLINICAL LABORATORY ANALYSIS, 2022, 36 (03)
  • [6] Mortality prediction of patients with spontaneous intracerebral hemorrhage by serum soluble Fas ligand concentrations
    Lorente, Leonardo
    Martin, Maria M.
    Perez-Cejas, Antonia
    Gonzalez-Rivero, Agustin F.
    Ramos-Gomez, Luis
    Sole-Violan, Jordi
    Caceres, Juan J.
    Cabrera, Judith
    Uribe, Luis
    Ferrer-Moure, Carmen
    Jimenez, Alejandro
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2022, 22 (02) : 233 - 238
  • [7] A Prognostic Marker in COVID-19 Disease Severity and Mortality: D-Dimer/Platelet Ratio
    Tahmaz, Alper
    Keskin, Aysegul Seremet
    Kizilates, Filiz
    CUREUS JOURNAL OF MEDICAL SCIENCE, 2023, 15 (05)
  • [8] Sysmex-derived COVID-19 prognostic score as an early prognostic marker for severity of the COVID-19 disease
    Blazek, Martin
    Vrbacky, Filip
    Fatorova, Ilona
    Mirska, Klara
    Zak, Pavel
    INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2024, 46 (02) : 243 - 249
  • [9] Predictors of severity and mortality in COVID-19 patients
    Hebatallah Hany Assal
    Hoda M. Abdel-hamid
    Sally Magdy
    Maged Salah
    Asmaa Ali
    Rasha Helmy Elkaffas
    Irene Mohamed Sabry
    The Egyptian Journal of Bronchology, 2022, 16
  • [10] Matrix metalloproteinase/Fas ligand (MMP/FasL) interaction dynamics in COVID-19: An in silico study and neuroimmune perspective
    Saleki, Kiarash
    Aram, Cena
    Alijanizadeh, Parsa
    Khanmirzaei, Mohammad Hossein
    Vaziri, Zahra
    Ramzankhah, Mohammad
    Azadmehr, Abbas
    HELIYON, 2024, 10 (10)