共 50 条
Development of chloroplast transformation and gene expression regulation technology in land plants
被引:5
|作者:
An, Yaqi
[1
,2
,3
,4
]
Wang, Yue
[1
,2
,3
,4
]
Wang, Xinwei
[5
]
Xiao, Jianwei
[1
,2
,3
,4
]
机构:
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China
[2] Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamental, Minist Educ, Beijing, Peoples R China
[3] Beijing Forestry Univ, Tree & Ornamental Plant Breeding & Biotechnol Lab, Beijing, Peoples R China
[4] Beijing Forestry Univ, Grassland Adm, Beijing, Peoples R China
[5] Hebei North Univ, Coll Agr & Forestry, Zhangjiakou, Peoples R China
来源:
FRONTIERS IN PLANT SCIENCE
|
2022年
/
13卷
基金:
中国国家自然科学基金;
关键词:
chloroplast genome;
gene expression;
transformation;
aptamer;
dPPR;
CRISPR;
Cas;
PENTATRICOPEPTIDE REPEAT PROTEIN;
GUIDE RNA;
CHLAMYDOMONAS;
GROWTH;
CAS9;
DNA;
D O I:
10.3389/fpls.2022.1037038
中图分类号:
Q94 [植物学];
学科分类号:
071001 ;
摘要:
Chloroplasts in land plants have their own small circular DNA that is presumed to have originated from cyanobacteria-related endosymbionts, and the chloroplast genome is an attractive target to improve photosynthetic ability and crop yield. However, to date, most transgenic or genetic engineering technologies for plants are restricted to manipulations of the nuclear genome. In this review, we provide a comprehensive overview of chloroplast genetic engineering and regulation of gene expression from the perspective of history and biology, focusing on current and latest methods. In addition, we suggest techniques that may regulate the chloroplast gene expression at the transcriptional or post-transcriptional level.
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页数:7
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