Identification of triterpenoids and flavonoids, step-wise aeration treatment as well as antioxidant capacity of Glycyrrhiza uralensis Fisch cell

被引：22

作者：

Wang, Juan ^{[1
,3
]}

Zhang, Jian ^{[2
]}

Gao, Wenyuan ^{[1
,3
]}

Wang, Qin ^{[3
]}

Yin, Shuangshuang ^{[1
]}

Liu, Hui ^{[3
]}

Man, Shuli ^{[3
]}

机构：

[1] Tianjin Univ, Sch Pharmaceut Sci & Technol, Tianjin Key Lab Modern Drug Delivery & High Effic, Tianjin 300072, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, Sch Tradit Chinese Mat Med, Tianjin 300193, Peoples R China

[3] Tianjin Univ Sci & Technol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China

来源：

INDUSTRIAL CROPS AND PRODUCTS | 2013年 / 49卷

关键词：

Glycyrrhiza uralensis Fisch; Suspension cell; Triterpenoid saponin; Flavonoids; Aeration volume; Antioxidant; PANAX-GINSENG; ADVENTITIOUS ROOTS; SCALE-UP; BIOREACTOR; GROWTH; CULTURES; SAPONINS; DENSITY; VOLUME; OXYGEN;

D O I：

10.1016/j.indcrop.2013.05.021

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, suspension cells of Glycyrrhiza uralensis Fisch. have been successfully established. Liquiritin, licorice glycoside B, licorice saponin J(2), glycyrrhizic acid and licorice saponin B-2 were identified from G. uralensis cells on the basis of LC-MSn analysis. Step-wise aeration treatment was conducted using a 51 balloon-type bubble bioreactor (BTBB) to enhance cell density and metabolite production. The results showed that step-wise aeration of 0.6-0.4 vvm was optimal for the accumulation of triterpenoid saponins (2.58 mg l(-1)) and flavonoids (24.33 mg l(-1)) in G. uralensis cells. Growth kinetics was also conducted with cells at aeration volume of 0.6-0.4 vvm. This study also revealed that G. uralensis cells possessed a similar radical scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) test as native licorice had. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：675 / 681

页数：7

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