Persistence of Ebola Virus in Sterilized Wastewater

被引：70

作者：

Bibby, Kyle ^{[1
,2
]}

Fischer, Robert J. ^{[3
]}

Casson, Leonard W. ^{[1
]}

Stachler, Elyse ^{[1
]}

Haas, Charles N. ^{[4
]}

Munster, Vincent J. ^{[3
]}

机构：

[1] Univ Pittsburgh, Dept Civil & Environm Engn, Pittsburgh, PA 15261 USA

[2] Univ Pittsburgh, Dept Computat & Syst Biol, Pittsburgh, PA 15261 USA

[3] NIAID, NIH, Virol Lab, Div Intramural Res, Hamilton, MT 59840 USA

[4] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS | 2015年 / 2卷 / 09期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

ENVIRONMENT STATE; DISEASE; ENTEROVIRUSES; KNOWLEDGE; STABILITY; SURVIVAL; PATIENT; FLUIDS; IMPACT;

D O I：

10.1021/acs.estlett.5b00193

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In the wake of the ongoing 2014/2015 Ebola virus outbreak, significant questions regarding the appropriate handling of Ebola virus-contaminated liquid waste remain, including the persistence of Ebola virus in wastewater. To address these uncertainties, we evaluated the persistence of Ebola virus spiked in sterilized domestic sewage. The viral titer decreased approximately 99% within the first test day from an initial viral titer of 10(6) TCID50 mL(-1); however, it could not be determined if this initial rapid decrease was due to aggregation or inactivation of the viral particles. The subsequent viral titer decrease was less rapid, and infectious Ebola virus particles persisted for all 8 days of the test. The inactivation constant (k) was determined to be -1.08 (2.1 days for a 90% viral titer decrease). Due to experimental conditions, we believe these results to be an upper bound for Ebola virus persistence in wastewater. Wastewater composition is inherently heterogeneous; subsequently, we caution that interpretation of these results should be made within a holistic assessment, including the effects of wastewater composition, dilution, and potential exposure routes within wastewater infrastructure. While it remains unknown if Ebola virus may be transmitted via wastewater, these data demonstrate a potential exposure route to infectious Ebola virus via wastewater and emphasize the value of a precautionary approach to wastewater handling in an epidemic response.

引用

页码：245 / 249

页数：5

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