Enantioseparation of tartaric acid by ligand-exchange capillary electrophoresis using contactless conductivity detection

被引：15
作者：
Knob, Radim [1 ]
Petr, Jan [1 ]
Sevcik, Juraj [1 ]
Maier, Vitezslav [1 ]
机构：
[1] Palacky Univ, Dept Analyt Chem, Reg Ctr Adv Technol & Mat, Fac Sci, Olomouc 77146, Czech Republic
来源：
JOURNAL OF SEPARATION SCIENCE | 2013年 / 36卷 / 20期
关键词：
Capillary electrophoresis; Contactless conductivity detection; Enantioseparation; Ligand exchange; Tartaric acid; DIRECT CHIRAL RESOLUTION; AMINO-ACIDS; ALPHA-HYDROXY; ENANTIOMERS; SEPARATION; CE; CU(II);
D O I：
10.1002/jssc.201300507
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu(II) as a central metal ion together with l-hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl2, 14 mM trans-4-hydroxy-l-proline, and 100 mM epsilon-aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide-coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from epsilon-aminocaproic acid to l-histidine. With detection limits of about 20 M, the method was applied to the analysis of wine and grape samples; only l-tartaric acid was found.
引用
收藏
页码：3426 / 3431
页数：6
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