Preparation of a porous conductive scaffold from aniline pentamer-modified polyurethane/PCL blend for cardiac tissue engineering

被引:104
作者
Baheiraei, Nafiseh [1 ,2 ]
Yeganeh, Hamid [3 ]
Ai, Jafar [1 ]
Gharibi, Reza [3 ]
Ebrahimi-Barough, Somayeh [1 ]
Azami, Mahmoud [1 ]
Vahdat, Sadaf [4 ,5 ]
Baharvand, Hossein [4 ]
机构
[1] Univ Tehran Med Sci, Dept Tissue Engn & Appl Cell Sci, Sch Adv Med Technol, Tehran 1417755469, Iran
[2] ACECR, Avicenna Res Inst, Dept Tissue Engn, Reprod Biotechnol Res Ctr, Tehran, Iran
[3] Iran Polymer & Petrochem Inst, Dept Polyurethane, Tehran, Iran
[4] ACECR, Dept Stem Cells & Dev Biol, Cell Sci Res Ctr, Royan Inst Stem Cell Biol & Technol, Tehran, Iran
[5] Univ Tehran, Coll Sci, Dept Biotechnol, Tehran, Iran
基金
美国国家科学基金会;
关键词
tissue engineering; cardiomyocytes; conducting polymers; polyurethane; cardiac patch; CELL-DERIVED CARDIOMYOCYTES; BIODEGRADABLE POLYURETHANE; POLYMER SCAFFOLDS; POLYPYRROLE; POLYLACTIDE; POLYANILINE; FABRICATION; STIMULATION; COPOLYMER; POROGEN;
D O I
10.1002/jbm.a.35447
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A novel biodegradable electroactive polyurethane containing aniline pentamer (AP) was blended with polycaprolactone (PCL). The prepared blend (PB) and PCL were further fabricated in to scaffolds using a mixture of poly(ethylene glycol) and salt particles in a double porogen particulate leaching and compression molding methodology. Scaffolds held open and interconnected pores having pore size ranging from several m to 150 mu m. PB scaffolds had compression modulus and strength of 4.1 and 1.3 MPa, respectively. The conductivity of the scaffold was measured as 10(-5) +/- 0.09 S.cm(-1) and preserved for at least 100 h post fabrication. Scaffolds supported neonatal cardiomyocytes adhesion and growth with PB showing more extensive effect on the expression of the cardiac genes involved in muscle contraction and relaxation (troponin-T) and cytoskeleton alignment (actinin-4). Our results highlight the potential of incorporation of AP as an electroactive moiety for induction of cardiomyocyte proliferation and repair of damaged heart tissue. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3179-3187, 2015.
引用
收藏
页码:3179 / 3187
页数:9
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