Detection of nitric oxide in tissue by spin trapping EPR spectroscopy and triacetylglycerol extraction

被引：20

作者：

Zhou, GY ^{[1
]}

Zhao, BL ^{[1
]}

Hou, JW ^{[1
]}

Li, MF ^{[1
]}

Chen, C ^{[1
]}

Xin, WJ ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 08期

基金：

中国国家自然科学基金;

关键词：

adriamycin; EPR; nitric oxide; spin trapping; triacetylglycerol;

D O I：

10.1023/A:1008977314541

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified method based on EPR spin trapping and triacetylglycerol extraction was used for tissue nitric oxide (NO) detection at room temperature. NO signal intensity was stable for about 1.5 h and the detection limit of this method was less than 200 pmol g(-1) tissue. Using this method, we report evidence that NO production in vivo can be inhibited by adriamycin in mice livers.

引用

页码：507 / 511

页数：5

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