Analysis of histological and microRNA profiles changes in rabbit skin development

被引：9

作者：

Ding, Haisheng ^{[1
]}

Cheng, Guanglong ^{[1
]}

Leng, Jianjian ^{[1
]}

Yang, Yongxin ^{[1
]}

Zhao, Xiaowei ^{[1
]}

Wang, Xiaofei ^{[1
]}

Qi, Yunxia ^{[1
]}

Huang, Dongwei ^{[1
]}

Zhao, Huiling ^{[1
]}

机构：

[1] Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Anhui Key Lab Livestock & Poultry Prod Safety Eng, Hefei 230031, Peoples R China

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

HAIR LENGTH; STEM-CELLS; SMALL RNAS; NICHE; FGF5; RESOURCE; PROGENY; GENES; CYCLE; TOOL;

D O I：

10.1038/s41598-019-57327-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The periodic regrowth of rabbit fur is economically important. Here, we aimed to characterise the histological traits and microRNA (miRNA) expression profiles in the skin tissue of Wan Strain Angora rabbits at different weeks after plucking. Haematoxylin-eosin staining showed that hair follicles were in the telogen phase in the first week, while they were in the anagen phase from the fourth to twenty-fourth weeks. In addition, two small RNA libraries derived from skin samples of Wan Strain Angora rabbits at telogen and anagen stages yielded over 24 million high-quality reads. Specifically, 185 miRNAs were differentially expressed between the telogen and anagen phases. The function of the differentially expressed miRNAs was explored by comparing them with known mammalian miRNAs and by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis of their predicted targets. Five new functional miRNAs were validated using quantitative real-time PCR. Moreover, the fibroblast growth factor 5 (FGF5) gene was verified to be a target of conservative_NC_013672.1_9290 and conservative_NC_013675.1_10734. We investigated differential miRNA profiles between the telogen and anagen phases of the hair cycle and our findings provide a basis for future studies focusing on the mechanisms of miRNA-mediated regulation of rabbit hair follicle cycling.

引用

页数：9

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