The anti MRSA biofilm activity of Thymus vulgaris essential oil in nanovesicles

被引:37
|
作者
Paula Perez, Ana [1 ]
Perez, Noelia [1 ]
Suligoy Lozano, Carlos Mauricio [2 ]
Julia Altube, Maria [1 ]
de Farias, Marcelo Alexandre [3 ]
Portugal, Rodrigo Villares [3 ]
Buzzola, Fernanda [2 ]
Jose Morilla, Maria [1 ]
Lilia Romero, Eder [1 ]
机构
[1] Natl Univ Quilmes, Nanomed Res & Dev Ctr, Sci & Technol Dept, Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, Inst Invest Microbiol & Parasitol Med, Dept Microbiol Parasitol & Inmunol, IMPaM CONICET,Fac Med, Buenos Aires, DF, Argentina
[3] CNPEM, Brazilian Nanotechnol Natl Lab, Campinas, SP, Brazil
关键词
Thymus vulgaris essential oil; Nanovesicles; Antibiofilm activity; STAPHYLOCOCCUS-AUREUS; ARCHAEOSOMES; COMBINATION; ANTIFUNGAL; SPP;
D O I
10.1016/j.phymed.2018.12.025
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Background: Thymus vulgaris essential oil (T) could be an alternative to classical antibiotics against bacterial biofilms, which show increased tolerance to antibiotics and host defence systems and contribute to the persistence of chronic bacterial infections. Hypothesis: A nanovesicular formulation of T may chemically protect the structure and relative composition of its multiple components, potentially improving its antibacterial and antibiofilm activity. Study design: We prepared and structurally characterized T in two types of nanovesicles:nanoliposomes (L80-T) made of Soybean phosphatidylcholine (SPC) and Polysorbate 80 (P80) [SPC:P80:T 1:0.75:0.3 w:w], and nanoarchaeosomes (A80-T) made of SPC, P80 and total polar archaeolipids (TPA) extracted from archaebacteria Halorubrum tebenquichense [SPC:TPA:P80:T 0.5:0.50.75:0.7 w:w]. We determined the macrophage cytotoxicity and the antibacterial activity against Staphylococcus aureus ATCC 25,923 and four MRSA clinical strains. Results: L80-T (Z potential -4.1 +/- 0.6 mV, similar to 115 nm, similar to 22 mg/ml T) and A80-T (Z potential -6.6 +/- 1.5 mV, similar to 130 nm, similar to 42 mg/ml T) were colloidally and chemically stable, maintaining size, PDI, Z potential and T concentration for at least 90 days. While MIC90 of L80-T was> 4 mg/ml T, MIC90 of A80-T was 2 mg/ml T for all S. aureus strains. The antibiofilm formation activity was maximal for A80-T, while L80-T did not inhibit biofilm formation compared to untreated control. A80-T significantly decreased the biomass of preformed biofilms of S. aureus ATCC 25,923 strain and of 3 of the 4 clinical MRSA isolates at 4 mg/ml T. It was found that the viability of J774A. 1 macrophages was decreased significantly upon 24 h incubation with A80-T, L80-T and T emulsion at 0.4 mg/ml T. These results show that from 0.4 mg/ml T, a value lower than MIC90 and the one displaying antibiofilm activity, with independence of its formulation, T significantly decreased the macrophages viability. Conclusion: Overall, because of its lower MIC90 against planktonic bacteria, higher antibiofilm formation capacity and stability during storage, A80-T resulted better antibacterial agent than T emulsion and L80-T. These results open new avenues to explode the A80-T antimicrobial intracellular activity.
引用
收藏
页码:339 / 351
页数:13
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