Recent advances in ABE fermentation:: hyper-butanol producing Clostridium beijerinckii BA101

被引:102
作者
Qureshi, N [1 ]
Blaschek, HP [1 ]
机构
[1] Univ Illinois, Dept Food Sci & Human Nutr, Biotechnol & Bioengn Grp, Urbana, IL 61801 USA
关键词
Clostridium beijerinckii BA101; butanol; ABE; pilot plant; amylases; glucoamylases; pervaporation; economics;
D O I
10.1038/sj.jim.7000114
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This is an overview of the mutant strain Clostridium beijerinckii BA101 which produces solvents (acetone-butanol-ethanol, ABE) at elevated levels. This organism expresses high levels of amylases when grown on starch. C. beijerinckii BA101 hydrolyzes starch effectively and produces solvent in the concentration range of 27-29 g l(-1). C. beijerinckii BA101 has been characterized for both substrate and butanol inhibition. Supplementing the fermentation medium (MP2) with sodium acetate enhances solvent production to 33 g l(-1). The results of studies utilizing commercial fermentation medium and pilot plant-scale reactors are consistent with the results using small-scale reactors. Pervaporation, a technique to recover solvents, has been applied to fed-batch reactors containing C. beijerinckii BA101, and solvent production as high as 165 g l(-1) has been achieved. Immobilization of C. beijerinckii BA101 by adsorption and use in a continuous reactor resulted in reactor productivity of 15.8 g l(-1) h(-1). Recent economic studies employing C. beijerinckii BA101 suggested that butanol can be produced at US$0.20-0.25 lb(-1) by employing batch fermentation and distillative recovery. Application of new technologies such as pervaporation, fed-batch culture, and immobilized cell reactors is expected to further reduce these prices.
引用
收藏
页码:287 / 291
页数:5
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