Maturation of morphology, phenotype and functions of murine bone marrow-derived dendritic cells(DCs) induced by polysaccharide Kureha(PSK)

被引：5

作者：

Tan, Yonggang ^{[1
]}

Meng, Yiming ^{[2
]}

Wang, Zuozhou ^{[3
]}

Shan, Fengping ^{[2
,3
]}

Wang, Qiushi ^{[1
]}

Zhang, Ning ^{[4
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Oncol, Shenyang, Peoples R China

[2] China Med Univ, Sch Basic Med Sci, Dept Immunol, Shenyang, Peoples R China

[3] China Med Univ, Sch Basic Med Sci, Inst Pathol & Pathophysiol, Shenyang, Peoples R China

[4] China Med Univ, Shengjing Hosp, Dept Rheumatol & Immunol, Shenyang, Peoples R China

来源：

HUMAN VACCINES & IMMUNOTHERAPEUTICS | 2012年 / 8卷 / 12期

关键词：

polysaccharide kureha; bone marrow derived DCs; maturation; modulation; PROTEIN-BOUND POLYSACCHARIDE; TRAMETES-VERSICOLOR; VACCINE THERAPY; PROSTATE-CANCER; GENE-EXPRESSION; CELL-GROWTH; NK-CELLS; T-CELLS; K PSK; KRESTIN;

D O I：

10.4161/hv.21993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this work was to evaluate the influence of protein-bound polysaccharide Kureha (PSK) on murine dendritic cells (DCs). These impacts of PSK on DCs from bone marrow derived DCs (BMDCs) were assessed with inverted phase contrast microscope, conventional scanning electron microscopy (SEM), transmission electron microscopy (TEM) for morphology, fluorescence activated cell sorting (FACS) analysis, cytochemistry assay for key surface molecules, FITC-dextran for phagocytosis, bio-assay and enzyme linked immunosorbent assay (ELISA) for cytokine production. We found that under the influence of PSK, immature DCs changed into mature DCs with decrease of antigens up-taking, simultaneously high expression of key surface molecules of the MHC classII, CD40, CD80, CD86 and CD83 as well as more production of IL-12p70 and tumor necrosis factor alpha (TNF-alpha). These data indicate that PSK could markedly promote maturation of DCs and this adjuvant-like activity may have potential therapeutic value in vaccine preparation.

引用

页码：1808 / 1816

页数：9

共 42 条

[1] PSK and OK-432-induced immunomodulation of inducible nitric oxide (NO) synthase gene expression in mouse peritoneal polymorphonuclear leukocytes and NO-mediated cytotoxicity [J].

Asai, K ;

Kato, H ;

Hirose, K ;

Akaogi, K ;

Kimura, S ;

Mukai, S ;

Inoue, M ;

Yamamura, Y ;

Sano, H ;

Sugino, S ;

Yoshikawa, T ;

Kondo, M .

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2000, 22 (02) :221-235

[2]

Banchereau J, 2001, CANCER RES, V61, P6451

[3] Polysaccharopeptides of Coriolus versicolor:: physiological activity, uses, and production [J].

Cui, T ;

Chisti, Y .

BIOTECHNOLOGY ADVANCES, 2003, 21 (02) :109-122

[4]

Fisher M, 2002, ANTICANCER RES, V22, P1737

[5] Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age [J].

Garbe, Katharina ;

Bratke, Kai ;

Wagner, Stefanie ;

Virchow, Johann Christian ;

Lommatzsch, Marek .

HUMAN IMMUNOLOGY, 2012, 73 (05) :493-497

[6]

Harada M, 1997, INT J CANCER, V70, P362, DOI 10.1002/(SICI)1097-0215(19970127)70:3<362::AID-IJC19>3.3.CO

[7]

2-U

[8] Protein-bound polysaccharide K induced apoptosis of the human Burkitt lymphoma cell line, Namalwa [J].

Hattori, TS ;

Komatsu, N ;

Shichijo, S ;

Itoh, K .

BIOMEDICINE & PHARMACOTHERAPY, 2004, 58 (04) :226-230

[9] The roles of IL-12 and IL-23 in CD8+ T cell-mediated immunity against Listeria monocytogenes: Insights from a DC vaccination model [J].

Henry, Curtis J. ;

Grayson, Jason M. ;

Brzoza-Lewis, Kristina L. ;

Mitchell, Latoya M. ;

Westcott, Marlena M. ;

Cook, Anne S. ;

Hiltbold, Elizabeth M. .

CELLULAR IMMUNOLOGY, 2010, 264 (01) :23-31

[10] Acute and subchronic oral toxicity of Coriolus versicolor standardized water extract in Sprague-Dawley rats [J].

Hor, Sook Yee ;

Ahmad, Mariam ;

Farsi, Elham ;

Lim, Chung Pin ;

Asmawi, Mohd. Zaini ;

Yam, Mun Fei .

JOURNAL OF ETHNOPHARMACOLOGY, 2011, 137 (03) :1067-1076

← 1 2 3 4 5 →