Long noncoding RNA PCAT-1 acts as an oncogene in osteosarcoma by reducing p21 levels

被引：40
作者：
Huang, Jiang [1 ]
Deng, Gaorong [2 ]
Liu, Tianmi [3 ]
Chen, Wenzhao [1 ]
Zhou, Yang [1 ]
机构：
[1] Nanchang Univ, Affiliated Hosp 1, Dept Orthopaed Surg, 17 Yong Waizheng St, Nanchang 330006, Jiangxi, Peoples R China
[2] Nanchang Univ, Affiliated Hosp 4, Dept Orthopaed Surg, Nanchang 330009, Jiangxi, Peoples R China
[3] Nanchang Univ, Affiliated Hosp 1, Dept Rehabil Med, Nanchang 330006, Jiangxi, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 495卷 / 04期
关键词：
LncRNA PCAT-1; Osteosarcoma; Cell proliferation; p21; EXPRESSION PROMOTES PROLIFERATION; HEPATOCELLULAR-CARCINOMA; PROSTATE-CANCER; COLORECTAL-CANCER; POOR-PROGNOSIS; GASTRIC-CANCER; REPRESSES P21; METASTASIS; CELLS; OVEREXPRESSION;
D O I：
10.1016/j.bbrc.2017.12.157
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Long non-coding RNA (lncRNA) is emerging as a critical regulator in multiple cancers. Recently, lncRNA PCAT-1 was found to be up-regulated in prostate cancer and hepatocellular carcinoma, exerting oncogenic effects. However, the biological function and regulatory mechanism of PCAT-1 remain unclear in osteosarcoma (OS). In this study, we reported that PCAT-1 expression was also upregulated in OS tissues, and its overexpression was remarkably associated with tumor size, Enneking stage, tumor node metastasis (TNM) stage and metastasis in patients with OS. Knockdown of PCAT-1 suppressed OS cells proliferation, migration and invasion in vitro, and inhibited the tumorigenicity of OS cells in vivo. Mechanistic investigations revealed that PCAT-1 could interact with EZH2, thereby repressing p21 expression. Additionally, rescue experiments indicated that PCAT-1 functioned as an oncogene partly via suppressing p21 in OS cells. Collectively, our findings demonstrate that PCAT-1 is a new candidate for use in OS diagnosis, prognosis and therapy. (C) 2017 Published by Elsevier Inc.
引用
收藏
页码：2622 / 2629
页数：8
相关论文
共 31 条
[1]   p21 in cancer: intricate networks and multiple activities [J].
Abbas, Tarek ;
Dutta, Anindya .
NATURE REVIEWS CANCER, 2009, 9 (06) :400-414
[2]   Long non-coding RNA PCAT-1 over-expression promotes proliferation and metastasis in gastric cancer cells through regulating CDKN1A [J].
Bi, Mingjun ;
Yu, Hongmei ;
Huang, Bin ;
Tang, Cuiyan .
GENE, 2017, 626 :337-343
[3]   Prognostic factors in high-grade osteosarcoma of the extremities or trunk:: An analysis of 1,702 patients treated on neoadjuvant cooperative osteosarcoma study group protocols [J].
Bielack, SS ;
Kempf-Bielack, B ;
Delling, G ;
Exner, GU ;
Flege, S ;
Helmke, K ;
Kotz, R ;
Salzer-Kuntschik, M ;
Werner, M ;
Winkelmann, W ;
Zoubek, A ;
Jürgens, H ;
Winkler, K .
JOURNAL OF CLINICAL ONCOLOGY, 2002, 20 (03) :776-790
[4]   Recent advances in osteosarcoma [J].
Botter, Sander M. ;
Neri, Dario ;
Fuchs, Bruno .
CURRENT OPINION IN PHARMACOLOGY, 2014, 16 :15-23
[5]   Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression [J].
Chen, Zhenyao ;
Chen, Xin ;
Chen, Ping ;
Yu, Shanxun ;
Nie, Fengqi ;
Lu, Binbin ;
Zhang, Te ;
Zhou, Yue ;
Chen, Qinnan ;
Wei, Chenchen ;
Wang, Wei ;
Wang, Zhaoxia .
CELL DEATH & DISEASE, 2017, 8 :e3092-e3092
[6]   Long noncoding RNA HOXA-AS2 represses P21 and KLF2 expression transcription by binding with EZH2, LSD1 in colorectal cancer [J].
Ding, J. ;
Xie, M. ;
Lian, Y. ;
Zhu, Y. ;
Peng, P. ;
Wang, J. ;
Wang, L. ;
Wang, K. .
ONCOGENESIS, 2017, 6 :e288-e288
[7]   Lost in transcription: p21 repression, mechanisms, and consequences [J].
Gartel, AL ;
Radhakrishnan, SK .
CANCER RESEARCH, 2005, 65 (10) :3980-3985
[8]   Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer [J].
Ge, Xiaosong ;
Chen, Yuanbin ;
Liao, Xiaoyu ;
Liu, Deqing ;
Li, Fangfang ;
Ruan, Honglian ;
Jia, Weihua .
MEDICAL ONCOLOGY, 2013, 30 (02)
[9]   Decapping of Long Noncoding RNAs Regulates Inducible Genes [J].
Geisler, Sarah ;
Lojek, Lisa ;
Khali, Ahmad M. ;
Baker, Kristian E. ;
Coller, Jeff .
MOLECULAR CELL, 2012, 45 (03) :279-291
[10]   The hallmarks of cancer A long non-coding RNA point of view [J].
Gutschner, Tony ;
Diederichs, Sven .
RNA BIOLOGY, 2012, 9 (06) :703-719
1234