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Evidence for glucocorticoid regulation of the rat vasopressin V-1a receptor gene
被引:9
作者:
Watters, JJ
Swank, MW
Wilkinson, CW
Dorsa, DM
机构:
[1] UNIV WASHINGTON,DEPT PSYCHIAT,SEATTLE,WA 98195
[2] FURMAN UNIV,DEPT PSYCHOL,GREENVILLE,SC 29613
[3] SEATTLE AMER LAKE VET ADM MED CTR,GRECC,SEATTLE,WA
[4] SEATTLE AMER LAKE VET ADM MED CTR,GRECC,TACOMA,WA
来源:
关键词:
glucocorticoids;
WRK-1;
cells;
liver;
adrenalectomy;
transcriptional regulation;
aldosterone;
glucocorticoid response element;
messenger RNA;
receptor binding;
D O I:
10.1016/0196-9781(95)02085-3
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
WRK-1 cells express vasopressin V-1a receptors. Twenty-four-hour treatment of these cells with dexamethasone (DEX) resulted in an increase in [H-3]AVP binding that was maximal at 12 h, and could be blocked by addition of RU 38486. The increases in [H-3]AVP binding were paralleled by increases in V-1a receptor mRNA. The in vivo effects of glucocorticoids (GCs) on V-1a receptor binding in hepatic tissue were also investigated in adrenalectomized and hormone-replaced rats given either DEX or aldosterone (ALDO). DEX effectively increased V-1a receptor binding site density whereas ALDO had no effect. The DEX effects on V-1a receptor mRNA and binding strongly implicate glucocorticoids in the regulation of V-1a receptor gene expression.
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页码:67 / 73
页数:7
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