Species variation in the spontaneous calcification of bone marrow-derived mesenchymal stem cells

被引：18

作者：

Huang, Yi-Zhou ^{[1
]}

Cai, Jia-Qin ^{[1
]}

Lv, Feng-Juan ^{[2
]}

Xie, Hong-Lei ^{[1
]}

Yang, Zhi-Ming ^{[1
]}

Huang, Yong-Can ^{[2
]}

Deng, Li ^{[1
]}

机构：

[1] Sichuan Univ, W China Hosp, Regenerat Med Res Ctr, Lab Stem Cell & Tissue Engn, Chengdu 610041, Peoples R China

[2] Univ Hong Kong, Li Ka Shing Fac Med, Dept Orthopaed & Traumatol, Hong Kong 999077, Hong Kong, Peoples R China

来源：

CYTOTHERAPY | 2013年 / 15卷 / 03期

基金：

国家高技术研究发展计划(863计划);

关键词：

bone marrow; mesenchymal stem cells; species variation; spontaneous calcification; OSTEOGENIC DIFFERENTIATION; STROMAL CELLS; TRANSPLANTATION; PROLIFERATION; CAPACITY;

D O I：

10.1016/j.jcyt.2012.11.011

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs.

引用

页码：323 / 329

页数：7

共 25 条

[1] Human bone marrow-derived mesenchymal stem cells do not undergo transformation after long-term In vitro culture and do not exhibit telomere maintenance mechanisms [J].