A novel nanocomposite for bone tissue engineering based on chitosan-silk sericin/hydroxyapatite: biomimetic synthesis and its cytocompatibility

被引:43
作者
Chen, Li [1 ,2 ]
Hu, Jingxiao [1 ]
Ran, Jiabing [1 ]
Shen, Xinyu [1 ]
Tong, Hua [1 ]
机构
[1] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China
[2] Wuhan Univ Sci & Technol, City Coll, Dept Urban Construct, Wuhan 430083, Peoples R China
基金
中国国家自然科学基金;
关键词
TROPICAL TASAR SILKWORM; IN-SITU PRECIPITATION; PROTEIN SERICIN; OXIDATIVE STRESS; CROSS-LINKING; STEM-CELLS; SCAFFOLDS; HYDROGELS; HYDROXYAPATITE; COLLAGEN;
D O I
10.1039/c5ra08216a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A simple and effective approach was developed to synthesize chitosan-silk sericin/hydroxyapatite nanocomposites by in situ precipitation and two methods of alkali diffusion were carried out in this study. The objective of this paper was to investigate the different properties of the nanocomposites. SEM showed that the rod-like hydroxyapatite particles with a diameter of 20-50 nm were distributed homogeneously within the chitosan-silk sericin matrix, and the formation mechanism was also investigated. The results of FTIR and XRD indicated that the inorganic phase in the nanocomposite was carbonate-substituted hydroxyapatite with low crystallinity. In terms of mechanical properties, chitosan-silk sericin/hydroxyapatite nanocomposites exhibited a higher elastic modulus and compressive strength than that of the chitosan/hydroxyapatite nanocomposites. In vitro cytocompatibility of the nanocomposite was evaluated by CCK-8 assay and SEM through MG63 osteoblast cells cultured on the samples, which demonstrated that they are non-toxic and support cell growth. These results suggest that the chitosan-silk sericin/hydroxyapatite nanocomposites are promising biomaterials for bone tissue engineering.
引用
收藏
页码:56410 / 56422
页数:13
相关论文
共 85 条
[11]   Composite polymer systems with control of local substrate elasticity and their effect on cytoskeletal and morphological characteristics of adherent cells [J].
Chou, Szu-Yuan ;
Cheng, Chao-Min ;
LeDuc, Philip R. .
BIOMATERIALS, 2009, 30 (18) :3136-3142
[12]   Cost comparison of anterior cruciate ligament reconstruction: Autograft versus allograft [J].
Cole, DW ;
Ginn, TA ;
Chen, GJ ;
Smith, BP ;
Curl, WW ;
Martin, DF ;
Poehling, GG .
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY, 2005, 21 (07) :786-790
[13]   Fabrication of sericin nanoparticles for controlled gene delivery [J].
Das, Saroj Kumar ;
Dey, Tuli ;
Kundu, S. C. .
RSC ADVANCES, 2014, 4 (05) :2137-2142
[14]   Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts [J].
Dash, Rupesh ;
Acharya, Chitrangada ;
Bindu, P. C. ;
Kundu, S. C. .
BMB REPORTS, 2008, 41 (03) :236-241
[15]   Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes [J].
Dash, Rupesh ;
Mandal, Mahitosh ;
Ghosh, Sudip K. ;
Kundu, S. C. .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2008, 311 (1-2) :111-119
[16]   Purification and biochemical characterization of a 70 kDa sericin from tropical tasar silkworm, Antheraea mylitta [J].
Dash, Rupesh ;
Ghosh, Sudip K. ;
Kaplan, David L. ;
Kundu, S. C. .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2007, 147 (01) :129-134
[17]   Freeze-casting of porous ceramics: A review of current achievements and issues [J].
Deville, Sylvain .
ADVANCED ENGINEERING MATERIALS, 2008, 10 (03) :155-169
[18]   Structure of genipin in solution: a combined experimental and theoretical study [J].
Di Tommaso, Stefania ;
David, Priscilla ;
Picolet, Karine ;
Gabant, Marion ;
David, Herve ;
Morancais, Jean-Luc ;
Gomar, Jerome ;
Leroy, Frederic ;
Adamo, Carlo .
RSC ADVANCES, 2013, 3 (33) :13764-13771
[19]   Sericin Enhances the Bioperformance of Collagen-Based Matrices Preseeded with Human-Adipose Derived Stem Cells (hADSCs) [J].
Dinescu, Sorina ;
Galateanu, Bianca ;
Albu, Madalina ;
Cimpean, Anisoara ;
Dinischiotu, Anca ;
Costache, Marieta .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (01) :1870-1889
[20]  
Elliott JC., 1994, STRUCTURE CHEM APATI, DOI DOI 10.1016/B978-0-444-81582-8.50008-0